caa a22 4500 469081783 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338052 doi (NATIONALLICENCE)springer-10.1007/BF00338052 Role of vitronectin in embryonic rat endocardial cell migration in vitro [Elektronische Daten] [Hiroshi Sumida, Harukazu Nakamura, Mineo Yasuda] Summary: Vitronectin is one of the extracellular matrices that mediate cell spreading and attachment in vitro. In the present paper, we demonstrate the involvement of vitronectin in the migration of cushion mesenchymal cells of the embryonic rat heart. Immunohistochemistry established the localization of vitronectin in the myocardial cells and in some of the cushion mesenchymal cells of the truncus arteriosus and atrioventricular canal. In vitro, vitronectin, fibronectin, and collagen type-I revcaled significant stimulating activity for cushion mesenchymal cell migration. The distance migrated by cushion mesenchymal cells cultured on vitronectin, collagen type-I, or both vitronectin and fibronectin was similar, but that on fibronectin was significantly shorter. Following the addition of anti-vitronectin IgG to the medium, the migration distance of cushion mesenchymal cells on fibronectin was remarkably increased. Most explants on vitronectin or on both vitronectin and fibronectin became detached from dishes after the addition of the antivitronectin antibody. Immunostaining revealed that cushion mesenchymal cells cultured on substrata other than vitronectin synthesized vitronectin. From these results, it is suggested that vitronectin is synthesized by myocardial cells and some cushion mesenchymal cells, and that vitronectin inhibits cell movement on fibronectin. This feature of vitronectin may be important in the regulation of the migration of cushion mesenchymal cell in vivo. Springer-Verlag, 1992 Vitronectin nationallicence Endocardial cells nationallicence Cell migration nationallicence Heart nationallicence Rat (Donryu) nationallicence Sumida Hiroshi Department of Anatomy, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, 734, Hiroshima, Japan aut Nakamura Harukazu Department of Biology, Kyoto Prefectural University of Medicine, 13 Nishitakatsukasa-cho Taishogun, Kita-ku, 603, Kyoto, Japan aut Yasuda Mineo Department of Anatomy, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, 734, Hiroshima, Japan aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 41-49 0302-766X 268:1<41 1992 268 441 https://doi.org/10.1007/BF00338052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sumida Hiroshi Department of Anatomy, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 700 1- Nakamura Harukazu Department of Biology, Kyoto Prefectural University of Medicine, 13 Nishitakatsukasa-cho Taishogun, Kita-ku, 603, Kyoto, Japan aut NATIONALLICENCE 700 1- Yasuda Mineo Department of Anatomy, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 41-49 0302-766X 268:1<41 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer