caa a22 4500 469081813 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338049 doi (NATIONALLICENCE)springer-10.1007/BF00338049 CGRP-immunoreactive endocrine cell proliferation in normal and hypoxic rat lung studied by immunocytochemical detection of incorporation of 5′-bromodeoxyuridine [Elektronische Daten] [L. Montuenga, D. Springall, J. Gaer, R. Winter, L. Zhao, J. McBride, K. Taylor, G. Barer, J. Polak] Summary: We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within the cells which renders them more detectable, rather than by a real increase in proliferation. The incorporation of continuously infused 5′-bromodeoxyuridine (BrdU) into nuclei of CGRP-containing cells was studied by immunohistochemistry in the airway and respiratory epithelium of rats kept in a hypoxic (10% O2), normobaric conditions for 7 days and in normoxic, normobaric controls. Some CGRP-immunoreactive cells could also be labelled for BrdU. However, the ratio of the number of cells labelled with both CGRP and BrdU to the number of cells labelled with CGRP alone did not differ significantly between hypoxic and normoxic rats (7.1±0.7 and 6.1±1.2, respectively; mean±SEM; P=0.49). These data strongly suggest that CGRP-containing endocrine cells or their precursors do proliferate in adult rat lung, but that the proliferation is not increased significantly in hypoxia. Springer-Verlag, 1992 Lung nationallicence Endocrine cells nationallicence Calcitonin generalated peptide nationallicence Bromodeoxyuridine nationallicence Differentiation nationallicence Proliferation nationallicence Hypoxia nationallicence Rat (Wistar) nationallicence Montuenga L. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Springall D. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Gaer J. Department of Surgery, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Winter R. Department of Respiratory Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Zhao L. Department of Respiratory Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut McBride J. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Taylor K. Department of Surgery, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Barer G. Section of Experimental Medicine, University of Sheffield Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut Polak J. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 9-15 0302-766X 268:1<9 1992 268 441 https://doi.org/10.1007/BF00338049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montuenga L. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Springall D. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Gaer J. Department of Surgery, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Winter R. Department of Respiratory Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Zhao L. Department of Respiratory Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- McBride J. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Taylor K. Department of Surgery, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Barer G. Section of Experimental Medicine, University of Sheffield Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 700 1- Polak J. Department of Histochemistry, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 9-15 0302-766X 268:1<9 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer