caa a22 4500 469081848 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319146 doi (NATIONALLICENCE)springer-10.1007/BF00319146 Formation of neuroblastic layers in chicken retinospheroids: the fibre layer of Chievitz secludes AChE-positive cells from mitotic cells [Elektronische Daten] [Elmar Willbold, Paul Layer] Summary: The significance of the classical subdivision of the retinal primitive neuroepithelium into an outer and an inner neuroblastic layer by the transient fibre layer of Chievitz (LOC) is little understood. We examine here the formation of neuroblastic layers by regenerating fully laminated retinospheroids from dissociated cells of the embryonic chick eye margin in rotary culture. By tracing cellular processes with the fibre-specific F11-antibody in retinospheroids, we occasionally find, in addition to an outer and an inner plexiform layer, a cell-free F11-positive LOC homologue, subdividing the inner nuclear layer. Moreover, we demonstrate that the LOC precisely separates postmitotic AChE-positive cells of the inner retina from an AChE-negative outer part holding all BrdU-labelled mitotic cells. These in vitro data suggest that the inner neuroblastic layer is exclusively composed of AChE-positive cells, thus representing a primary differentiation zone of the retina. Springer-Verlag, 1992 Reaggregation culture nationallicence Retina, histogenesis nationallicence Pigment epithelium nationallicence F11-antigen nationallicence Acetylcholinesterase nationallicence Chicken nationallicence Willbold Elmar Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/IV, W-7400, Tübingen, Germany aut Layer Paul Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/IV, W-7400, Tübingen, Germany aut Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 401-408 0302-766X 268:3<401 1992 268 441 https://doi.org/10.1007/BF00319146 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319146 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Willbold Elmar Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/IV, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Layer Paul Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/IV, W-7400, Tübingen, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 401-408 0302-766X 268:3<401 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer