caa a22 4500 469081864 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319157 doi (NATIONALLICENCE)springer-10.1007/BF00319157 Immunocytochemical localization of collagen types I, III, IV, and fibronectin in the human dermis [Elektronische Daten] Modifications with ageing [L. Vitellaro-Zuccarello, R. Garbelli, V. Rossi] Summary: The distribution of collagen types I, III, IV, and of fibronectin has been studied in the human dermis by light and electron-microscopic immunocytochemistry, using affinity purified primary antibodies and tetramethylrhodamine isothiocyanate-conjugated secondary antibodies. Type I collagen was present in all collagen fibers of both papillary and reticular dermis, but collagen fibrils, which could be resolved as discrete entities, were labeled with different intensity. Type III collagen codistributed with type I in the collagen fibers, besides being concentrated around blood vessels and skin appendages. Coexistence of type I and type III collagens in the collagen fibrils of the whole dermis was confirmed by ultrastructural double-labelling experiments using colloidal immunogold as a probe. Type IV collagen was detected in all basement membranes. Fibronectin was distributed in patches among collagen fibers and was associated with all basement membranes, while a weaker positive reaction was observed in collagen fibers. Ageing caused the thinning of collagen fibers, chiefly in the recticular dermis. The labeling pattern of both type I and III collagens did not change in skin samples from patients of up to 79 years of age, but immunoreactivity for type III collagen increased in comparison to younger skins. A loss of fibronectin, likely related to the decreased morphogenetic activity of tissues, was observed with age. Springer-Verlag, 1992 Dermis nationallicence Collagen fibers nationallicence Extracellular matrix nationallicence Fibronectin nationallicence Immunocytochemistry nationallicence Man nationallicence Vitellaro-Zuccarello L. Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, Via Celoria 26, I-20133, Milan, Italy aut Garbelli R. Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, Via Celoria 26, I-20133, Milan, Italy aut Rossi V. Istituto di Clinica Dermosifilopatica I e Dermatologia Pediatrica, Milan, Italy aut Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 505-511 0302-766X 268:3<505 1992 268 441 https://doi.org/10.1007/BF00319157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vitellaro-Zuccarello L. Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, Via Celoria 26, I-20133, Milan, Italy aut NATIONALLICENCE 700 1- Garbelli R. Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, Via Celoria 26, I-20133, Milan, Italy aut NATIONALLICENCE 700 1- Rossi V. Istituto di Clinica Dermosifilopatica I e Dermatologia Pediatrica, Milan, Italy aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 505-511 0302-766X 268:3<505 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer