caa a22 4500 469081902 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319159 doi (NATIONALLICENCE)springer-10.1007/BF00319159 Differentiation of smooth muscle cells in the fetal rat testis and ovary: localization of alkaline phosphatase, smooth muscle myosin, F-actin, and desmin [Elektronische Daten] [Jorma Paranko, Lauri Pelliniemi] Summary: The histochemical demonstration of alkaline phosphatase (AP) activity and localization of smooth muscle myosin (SMM), F-actin, and desmin were carried out on frozen sections of testes and ovaries from 15-day-old fetal to newborn rats. The presence of immunocytochemically localized SMM and desmin was confirmed by Western blot analysis of proteins from isolated gonads. The development of smooth muscle cells was predominant in the testis. The first SMM-positive cells with an increasing intensity for F-actin and desmin appeared in the testicular tunica albuginea and around the testicular cords by the age of 16 days. A continuous layer of SMM- and F-actin-positive (but not uniformly desmin-positive) myoid cells was detected in the newborn testis. In the early gonads and in the newborn ovary, a majority of the interstitial cells expressed desmin, indicating that, in undifferentiated tissues, non-myogenic cells may also express desmin. During fetal development, male and female gonocytes showed a decrease in F-actin content but retained their high AP activity. In the cortex of the newborn rat ovary, the observed high AP activity and the presence of desmin may be associated with the postnatal histogenesis of the follicles. The presence of SMM-containing cells in the hilus of the ovary may be required for the demarcation of the ovary from the mesonephros by the constriction of the mesovarium. The occurrence of SMM-positive cells predominantly in male fetuses suggests that the development of the contractile cells in the fetal testis may be induced by testicular androgens. Springer-Verlag, 1992 Development, ontogenetic nationallicence Testis nationallicence Ovary nationallicence Muscle, smooth nationallicence Alkaline phosphatase nationallicence Myosin nationallicence Actin nationallicence Desmin nationallicence Rat (Sprague-Dawley) nationallicence Paranko Jorma Department of Anatomy, University of Turku, Kiinamyllynkatu 10, SF-20520, Turku, Finland aut Pelliniemi Lauri Laboratory of Electron Microscopy, University of Turku, Kiinamyllynkatu 10, SF-20520, Turku, Finland aut Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 521-530 0302-766X 268:3<521 1992 268 441 https://doi.org/10.1007/BF00319159 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319159 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paranko Jorma Department of Anatomy, University of Turku, Kiinamyllynkatu 10, SF-20520, Turku, Finland aut NATIONALLICENCE 700 1- Pelliniemi Lauri Laboratory of Electron Microscopy, University of Turku, Kiinamyllynkatu 10, SF-20520, Turku, Finland aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 521-530 0302-766X 268:3<521 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer