caa a22 4500 469081910 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319149 doi (NATIONALLICENCE)springer-10.1007/BF00319149 Immunocytochemical localization of β-1,3-glucan recognition protein in the silkworm, Bombyx mori [Elektronische Daten] [Masanori Ochiai, Teruo Niki, Masaaki Ashida] Summary: A monospecific antibody against β-1,3-glucan recognition protein (a 62 kDa protein) of the larval silkworm prophenoloxidase activating system was used to study the localization of the protein. Among tissues from 5th instar larvae, only hemocytes and plasma were shown to contain a 62 kDa polypeptide immunoreactive with the antibody. Ultra-thin sections of the hemocytes were stained by an indirect immunogold staining method. Labelling occurred in the granules and cytoplasm of granulocytes and in the spherules and cytoplasm of spherulocytes. It was most conspicuous in granules of granulocytes and uniformly labelled spherules of spherulocyte, whereas no labelling was evident in prohemocytes, plasmatocytes and oenocytoids. The results are discussed in relation to the mode of recognition of fungi as non-self in insect hemocoel. Springer-Verlag, 1992 Hemocytes nationallicence Hemolymph nationallicence Phenoloxidase nationallicence β-1,3-glucan nationallicence Bombyx mori (Insecta) nationallicence Ochiai Masanori Biochemical Laboratory, The Institute of Low Temperature Science, Hokkaido University, 060, Sapporo, Japan aut Niki Teruo Laboratory of Cellular Technology, Faculty of Technology, Takushoku University, Hachioji-shi, Tokyo, Japan aut Ashida Masaaki Biochemical Laboratory, The Institute of Low Temperature Science, Hokkaido University, 060, Sapporo, Japan aut Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 431-437 0302-766X 268:3<431 1992 268 441 https://doi.org/10.1007/BF00319149 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319149 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ochiai Masanori Biochemical Laboratory, The Institute of Low Temperature Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Niki Teruo Laboratory of Cellular Technology, Faculty of Technology, Takushoku University, Hachioji-shi, Tokyo, Japan aut NATIONALLICENCE 700 1- Ashida Masaaki Biochemical Laboratory, The Institute of Low Temperature Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 431-437 0302-766X 268:3<431 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer