caa a22 4500 469081945 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319154 doi (NATIONALLICENCE)springer-10.1007/BF00319154 Receptor-mediated and adsorptive endocytosis by male germ cells of different mammalian species [Elektronische Daten] [Dominique Segretain, Monique Egloff, Nadine Gérard, Charles Pineau, Bernard Jégou] Summary: The routes for adsorptive and receptor-mediated endocytosis were studied in vivo after microinjection of tracers into the lumen of the seminiferous tubules, and in vitro in isolated germ cells of different mammals. Cationic ferritin was located on the plasma membrane, in vesicles, in tubules, in multivesicular bodies and in lysosome-like granules of mouse spermatocytes. In these cells the number of multivesicular bodies varied during spermatogenesis. Spermatids and to a lesser extent residual bodies also performed adsorptive endocytosis. In the rat and monkey (Macaca fascicularis) diferric transferrin was specifically taken up by germ cells via receptor-mediated endocytosis. The labelling was observed subsequently in membrane pits, vesicles, endosome-like bodies and pale multivesicular bodies. A progressive decrease in the frequency of the labelling of the germ cells by transferrin-gold particles was observed from spermatogonia to spermatocytes and to early spermatids, which could indicate that iron is particularly required by germ cells during the mitotic and meiotic processes. Adsorptive and receptor-mediated endocytosis therefore occurs in all classes of germ cells. These endocytic processes are most probably required for germ cell division, differentiation and metabolism. Springer-Verlag, 1992 Testis nationallicence Endocytosis nationallicence Spermatogenesis nationallicence Spermatogonia nationallicence Spermatocytes nationallicence Spermatids nationallicence Transferrin nationallicence Ferritin nationallicence Sertoli cells nationallicence Mouse nationallicence Rat nationallicence Monkey, Macaca fascicularis (Primates) nationallicence Segretain Dominique Laboratoire d'Histologie-Embryologie, CHU Paris Ouest, 45, Rue des Saint-Pères, F-75006, Paris, France aut Egloff Monique Faculté de Médecine, Laboratoire Hospitalo-Universitaire, 45, Rue des Saint-Pères, F-75270, Paris, France aut Gérard Nadine Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut Pineau Charles Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut Jégou Bernard Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 471-478 0302-766X 268:3<471 1992 268 441 https://doi.org/10.1007/BF00319154 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319154 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Segretain Dominique Laboratoire d'Histologie-Embryologie, CHU Paris Ouest, 45, Rue des Saint-Pères, F-75006, Paris, France aut NATIONALLICENCE 700 1- Egloff Monique Faculté de Médecine, Laboratoire Hospitalo-Universitaire, 45, Rue des Saint-Pères, F-75270, Paris, France aut NATIONALLICENCE 700 1- Gérard Nadine Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut NATIONALLICENCE 700 1- Pineau Charles Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut NATIONALLICENCE 700 1- Jégou Bernard Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04, Université de Rennes 1, Campus de Beaulieu, F-35042, Rennes Cedex, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 471-478 0302-766X 268:3<471 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer