caa a22 4500 469081961 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00319155 doi (NATIONALLICENCE)springer-10.1007/BF00319155 Andersson Tommy Department of Zoophysiology, University of Göteborg, Box 25059, S-400 31, Göteborg, Sweden aut Subcellular fractionation of rainbow trout gonads with emphasis on microsomal enzymes involved in steroid metabolism [Elektronische Daten] [Tommy Andersson] Summary: Rainbow trout gonads were subfractionated by differential centrifugation with emphasis on obtaining preparations suitable for the study of steroid-metabolizing enzymes. A fractionation scheme was evaluated for the mature testis and for 3 ovarian developmental stages. The distribution of cell organelles among the fractions was determined using enzyme-markers and electron microscopy. The fractionation scheme was found to be suitable for separating mitochondria and microsomes which were recovered at similar yields to those that had been reported for other extraheptic fish tissues. Fractionation of the mature ovary was fraught with problems probably because a large yolk protein cytosole fraction interfered with the recovery of microsomes. However, no difference in the specific activity of microsomal NADPH-cytochrome c-reductase between the various organ preparations was evident. The testis microsomes contained detectable amounts of cytochrome P450, whereas its content in the various ovary microsomes was too low to be detected. Progesterone 17α-hydroxylase was detected in microsomes from testes and early developing ovaries, and microsomal aromatase activity was present in microsomes from early developing, mature and postovulatory ovaries. Furthermore, the testis microsomes contained a highly active UDP glucuronosyltransferase with testosterone used as a substrate. Springer-Verlag, 1992 Gonadal hormones nationallicence Microsomes nationallicence Steroid metabolism nationallicence Cytochrome P450 nationallicence UDP glucuronosyltransferase nationallicence Rainbow trout ( Oncorhynchus mykiss ) nationallicence Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 479-489 0302-766X 268:3<479 1992 268 441 https://doi.org/10.1007/BF00319155 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319155 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Andersson Tommy Department of Zoophysiology, University of Göteborg, Box 25059, S-400 31, Göteborg, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/3(1992-06-01), 479-489 0302-766X 268:3<479 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer