caa a22 4500 469082038 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318793 doi (NATIONALLICENCE)springer-10.1007/BF00318793 Distribution patterns and coexistence of neurohormonal peptides (ANP, BNP, NPY, SP, CGRP, enkephalins) in chromaffin cells and nerve fibers of the anuran adrenal organ [Elektronische Daten] [M. Reinecke, C. Heym, W. Forssmann] Summary: Immunohistochemical techniques were used to study the adrenal organs of the anuran species Rana esculenta, Caldula pulchra and Bufo marinus with respect to the distribution and coexistence of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), Leu-enkephalin (Leu-ENK). Met-enkephalin-Arg-Phe (MEAP) and dynorphin A 1-17 (DYN). Antisera against enzymes involved in catecholamine synthesis, i.e., dopamine-β-hydroxylase (DBH) and tyrosine hydroxylase (TH), were used for the identification of chromaffin cells. ANP-immunoreactive (-IR) cells occurred in high densities (30%-70% of the total cell population) in all species investigated. In C. pulchra and B. marinus, BNP-IR cells constituted a population of non-DBH-IR and non-TH-IR cells that were different from the ANP-IR cells. A large proportion of the adrenal cells (10%-55%) were immunoreactive to Leu-ENK, and a minority (2%-5%) showed MEAP-immunoreactivity. DYN-immunoreactivity was not observed. The anurans studied exhibited small numbers of SP-IR, CGRP-IR and NPY-IR cells. Immunoreactivities for ANP+Leu-ENK and Leu-ENK+ MEAP were shown to coexist. In C. pulchra and B. marinus, immunoreactions for ANP+NPY, ANP+SP and SP+CGRP were also colocalized. Except for DYN, all neurohormonal peptides also occurred in intra-adrenal nerve fibers. SP-IR fibers also displayed CGRP-immunoreactivity and some Leu-ENK-IR fibers contained MEAP-immunoreactivity. In C. pulchra, NPY-IR fibers were found that also showed ANP-immunoreactivity. Springer-Verlag, 1992 Adrenal organ nationallicence Atrial natriuretic peptides nationallicence Neuropeptide Y nationallicence Substance P nationallicence Calcitonin gene-related peptide nationallicence Enkephalins nationallicence Catecholamine synthesizing enzymes nationallicence Rand esculenta, Caldula pulchra, Bufo marinus (Anura) nationallicence Reinecke M. Institute of Anatomy, University of Zürich, Winterthurer Strasse 190, CH-8057, Zürich, Switzerland aut Heym C. Institut für Anatomie I der Universität, Im Neuenheimer Feld 307, W-6900, Heidelberg, Germany aut Forssmann W. Niedersächsisches Institut für Peptidforschung (IPF), Feodor-Lynen-Strasse 5, W-3000, Hannover 61, Germany aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 247-256 0302-766X 268:2<247 1992 268 441 https://doi.org/10.1007/BF00318793 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318793 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reinecke M. Institute of Anatomy, University of Zürich, Winterthurer Strasse 190, CH-8057, Zürich, Switzerland aut NATIONALLICENCE 700 1- Heym C. Institut für Anatomie I der Universität, Im Neuenheimer Feld 307, W-6900, Heidelberg, Germany aut NATIONALLICENCE 700 1- Forssmann W. Niedersächsisches Institut für Peptidforschung (IPF), Feodor-Lynen-Strasse 5, W-3000, Hannover 61, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 247-256 0302-766X 268:2<247 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer