caa a22 4500 469082070 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318800 doi (NATIONALLICENCE)springer-10.1007/BF00318800 Ultrastructural identification and distribution of the adhesion molecules ICAM-1 and LFA-1 in the vascular and extravascular compartments of the human palatine tonsil [Elektronische Daten] [M. Perry, K. Brown, B. von Gaudecker] Summary: Immunohistological analysis of sections prepared from human palatine tonsils revealed marked differences in the distribution of the adhesion molecule, leucocyte function antigen-1 (LFA-1) and its counter receptor, intercellular adhesion molecule-1 (ICAM-1). Light microscopy showed that LFA-1 was restricted to the leucocytes, particularly the lymphocytes. In contrast, staining of ICAM-1 was predominantly confined to the vascular endothelium with the greatest expression seen on the morphologically distinct high endothelial venules in the parafollicular areas; these are the sites that appear to support lymphocyte migration. Electron microscopy revealed that ICAM-1 was present on the luminal and lateral surfaces of the high endothelium and absent from the abluminal surface supported by basal lamina. The ICAM-1 was also absent from those surfaces of the endothelium that were in close contact with intravascular lymphocytes. Other cells stained by the anti-ICM-1 antibody included dendritic cells, plasma cells and epithelial cells in the reticulated crypt epithelium and in the upper strata of the non-keratinised stratified squamous epithelium. The high expression of LFA-1 was most prominent on lymphocytes, low on antigen-presenting cells and activated lymphoid cells, and not detectable on plasma cells, epithelial and endothelial cells. We propose that LFA-1/ICAM-1 binding participates in mediating the transendothelial migration of lymphocytes across the high endothelial venules of palatine tonsil. Springer-Verlag, 1992 Palatine tonsil nationallicence Immuno-electron microscopy nationallicence High endothelial venules nationallicence Adhesion molecules nationallicence ICAM-1, LFA-1 nationallicence Human nationallicence Perry M. Division of Anatomy and Cell Biology, UMDS, Guy's Hospital, London Bridge, SE1 9RT, London, UK aut Brown K. Department of Immunology, The Rayne Institute, UMDS, St. Thomas's Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut von Gaudecker B. Anatomisches Institut, Christian Albrechts Universität, Olshausenstrasse 40, W-2300, Kiel 1, Germany aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 317-326 0302-766X 268:2<317 1992 268 441 https://doi.org/10.1007/BF00318800 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318800 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perry M. Division of Anatomy and Cell Biology, UMDS, Guy's Hospital, London Bridge, SE1 9RT, London, UK aut NATIONALLICENCE 700 1- Brown K. Department of Immunology, The Rayne Institute, UMDS, St. Thomas's Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- von Gaudecker B. Anatomisches Institut, Christian Albrechts Universität, Olshausenstrasse 40, W-2300, Kiel 1, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 317-326 0302-766X 268:2<317 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer