caa a22 4500 469082089 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318801 doi (NATIONALLICENCE)springer-10.1007/BF00318801 Monoclonal antibodies against surface components of the mechano-and chemosensitive cnidocil complex of Hydra vulgaris [Elektronische Daten] [R. Golz, U. Thurm] Summary: Two monoclonal antibodies (Gc3.2 and Bd 2.2) against surface components of the cnidocil complex of Hydra vulgaris have been produced. In indirect immunofluorescence and in immunogold-labelling, the Gc 3.2-antibody stains the complete surface of all nematocytes, whereas other cellular surfaces are not labelled. The Bd 2.2-antibody, in contrast, produces only a small band of fluorescence on isolated cnidocils. This pattern of fluorescence and the corresponding immunogold-labelling indicate that the Bd 2.2-antibody exclusively binds to those intermembrane connectors that link the cnidocil and stereovillar cone in situ. In isolated and decnidociliated nematocytes, the tips of the stereovilli are also labelled by the Bd 2.2-antibody. Physiological experiments suggest that the Bd 2.2-antibody disturbs the reconstitution of intermembrane connectors during cnidocil regeneration. These data confirm the hypothesis that the intermembrane connectors are formed by two identical subunits located at the cnidociliar and stereovillar surfaces. Springer-Verlag, 1992 Cnidocil complex nationallicence Monoclonal antibodies nationallicence Intermembrane connectors nationallicence Cnidocil isolation nationallicence Hydra vulgaris (Cnidaria) nationallicence Golz R. Institut für Neuro- und Verhaltensbiologie, Hüfferstrasse 1, W-4400, Münster, Germany aut Thurm U. Institut für Neuro- und Verhaltensbiologie, Hüfferstrasse 1, W-4400, Münster, Germany aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 327-333 0302-766X 268:2<327 1992 268 441 https://doi.org/10.1007/BF00318801 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318801 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Golz R. Institut für Neuro- und Verhaltensbiologie, Hüfferstrasse 1, W-4400, Münster, Germany aut NATIONALLICENCE 700 1- Thurm U. Institut für Neuro- und Verhaltensbiologie, Hüfferstrasse 1, W-4400, Münster, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 327-333 0302-766X 268:2<327 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer