caa a22 4500 469082143 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318805 doi (NATIONALLICENCE)springer-10.1007/BF00318805 Intercellular communication between cultured granulosa cells of the marmoset ( Callithrix jacchus ) [Elektronische Daten] [G. Rune, D. Pretzer, W. Beuthe, H. Merker] Summary: The influence of follicle-stimulating hormone, forskolin, insulin-like growth factor type I, epidermal growth factor, and 12-tetradecanoyl-phorbol-13-acetate on marmoset granulosa cell communication via gap junctions was investigated by morphological means and microinjection of carboxyfluorescein. Gap junctions between neighbouring granulosa cells were present in all groups. The number, but not length, of gap junctions between marmoset granulosa cells increased when the cells had been treated with follicle-stimulating hormone, insulin-like growth factor type I, and follicle-stimulating hormone plus insulin-like growth factor type I. No effect on gap junctions was seen, after exposure of the cells to the other three substances. Carboxyfluorescein and counting of the surrounding labelled cells showed that supplementation with follicle-stimulating hormone, forskolin, insulin-like growth factor type I and epidermal growth factor from the beginning of cultivation led to an increase in stained cells after 48 h. When treatment was started in 48 h cultures the substances reached their maximal activity within 30 min (forskolin and epidermal growth factor) or 3 h (follicle-stimulating hormone and insulin-like growth factor type I). Spreading of the fluorescen dye was inhibited when the medium was supplemented with 12-tetradecanoyl-phorbol-13-acetate. This effect was maximal after 30 min. Additive effects regarding the coupling of the cells were seen by combining of epidermal growth factor with follicle-stimulating hormone, but not with insulin-like growth factor type I or forskolin plus follicle-stimulating hormone. Springer-Verlag, 1992 Ovary nationallicence Granulosa cells nationallicence Gap junctions nationallicence Microinjection nationallicence Marmoset, Callithrix jacchus (Primates) nationallicence Rune G. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut Pretzer D. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut Beuthe W. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut Merker H. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 369-376 0302-766X 268:2<369 1992 268 441 https://doi.org/10.1007/BF00318805 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318805 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rune G. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut NATIONALLICENCE 700 1- Pretzer D. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut NATIONALLICENCE 700 1- Beuthe W. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut NATIONALLICENCE 700 1- Merker H. Institut für Anatomie, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 369-376 0302-766X 268:2<369 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer