caa a22 4500 469082151 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318789 doi (NATIONALLICENCE)springer-10.1007/BF00318789 Progressive redistribution of alcohol dehydrogenase during vitellogenesis in Drosophila melanogaster [Elektronische Daten] characterization of ADH-positive bodies in mature oocytes [Neus Visa, Joan Fibla, Roser Gonzàlez-Duarte, María Santa-Cruz] Summary: The use of monoclonal antibodies against Drosophila alcohol dehydrogenase (ADH) provides a powerful tool in the analysis of the tissue and temporal patterns of Adh gene expression. Immunocytochemical techniques at the light- and electron-microscopic levels have been used to determine the distribution of ADH in the ovarian follicles of D. melanogaster during oogenesis. In the early stages of oogenesis, small amounts of ADH are detectable in the cystocytes. At the beginning of vitellogenesis (S7), ADH appears to be located mainly in the nurse cells. From stage S9 onwards, the ADH protein is evenly distributed in the ooplasm until the later stages of oogenesis (S13-14), when multiple ADH-positive bodies of varying size appear in the ooplasm. This change in distribution is a result of the compartmentalization of the ADH protein within the glycogen yolk or β-spheres. Yolk becomes enclosed within the lumen of the primitive gut during embryonic development, and thus our results suggest a mechanism for the transfer of maternally-inherited enzymes to the gut lumen via yolk spheres. Springer-Verlag, 1992 Alcohol dehydrogenase (ADH) nationallicence Oogenesis nationallicence Vitellogenesis nationallicence Yolk nationallicence Immunocytochemistry nationallicence Drosophila melanogaster (Insecta) nationallicence Visa Neus Unitat de Citologia i Histologia, Departament de Biologia Cellular i Fisiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193, Bellaterra/Barcelona, Spain aut Fibla Joan Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028, Barcelona, Spain aut Gonzàlez-Duarte Roser Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028, Barcelona, Spain aut Santa-Cruz María Unitat de Citologia i Histologia, Departament de Biologia Cellular i Fisiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193, Bellaterra/Barcelona, Spain aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 217-224 0302-766X 268:2<217 1992 268 441 https://doi.org/10.1007/BF00318789 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318789 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Visa Neus Unitat de Citologia i Histologia, Departament de Biologia Cellular i Fisiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193, Bellaterra/Barcelona, Spain aut NATIONALLICENCE 700 1- Fibla Joan Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Gonzàlez-Duarte Roser Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Santa-Cruz María Unitat de Citologia i Histologia, Departament de Biologia Cellular i Fisiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193, Bellaterra/Barcelona, Spain aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 217-224 0302-766X 268:2<217 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer