caa a22 4500 469082216 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318790 doi (NATIONALLICENCE)springer-10.1007/BF00318790 Morphological differentiation of endothelial cells co-cultured with astrocytes on type-I or type-IV collagen [Elektronische Daten] [Motoki Tagami, Kazuo Yamagata, Hideaki Fujino, Akiyoshi Kubota, Yasuo Nara, Yukio Yamori] Summary: In this study bovine aortic endothelial cells were co-cultured with astrocytes from fetal Wistar Kyoto rats. Endothelial cells growing on type-I collagen, development. Although some cells appeared to be mature, horseradish peroxidase penetrated within 1 min of incubation through the intercellular junctions of these endothelial elements maintained on type-I collagen. In contrast, endothelial cells on type-IV collagen, co-cultured with astrocytes, were well developed; their intercellular junctions were well established, and plasmalemmal vesicles reduced in number. As a result, horseradish peroxidase was unable to penetrate through the endothelial cells grown on type-IV collagen and co-cultured with astrocytes because of the reduced extent of the junctional and vesicular transport. These findings reveal that (1) type-IV collagen is essential for the differentiation of endothelial cells, (2) endothelial cell-astrocyte interactions occur during co-culture, and (3) endothelial permeability depends on astrocyte-produced factors, in addition to type-IV collagen. Springer-Verlag, 1992 Astrocytes nationallicence Endothelial cells nationallicence Type-I collagen nationallicence Type-IV collagen nationallicence Blood-brain barrier nationallicence Tight junctions nationallicence Cell culture: Bovine nationallicence Rat (Wistar Kyoto) nationallicence Tagami Motoki Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut Yamagata Kazuo Biotechnology Section, Sumitomo Metal Industry, 229, Sagamihara, Kanagawa, Japan aut Fujino Hideaki Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut Kubota Akiyoshi Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut Nara Yasuo Department of Pathology, Shimane Medical University, 693, Izumo, Japan aut Yamori Yukio Department of Pathology, Shimane Medical University, 693, Izumo, Japan aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 225-232 0302-766X 268:2<225 1992 268 441 https://doi.org/10.1007/BF00318790 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318790 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tagami Motoki Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamagata Kazuo Biotechnology Section, Sumitomo Metal Industry, 229, Sagamihara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Fujino Hideaki Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut NATIONALLICENCE 700 1- Kubota Akiyoshi Department of Medicine, Sanraku Hospital, Chiyoda-ku, 101, Tokyo, Japan aut NATIONALLICENCE 700 1- Nara Yasuo Department of Pathology, Shimane Medical University, 693, Izumo, Japan aut NATIONALLICENCE 700 1- Yamori Yukio Department of Pathology, Shimane Medical University, 693, Izumo, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 225-232 0302-766X 268:2<225 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer