caa a22 4500 469082224 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318802 doi (NATIONALLICENCE)springer-10.1007/BF00318802 Uptake and intracellular transport of cationic ferritin in the bronchiolar and alveolar epithelia of the rat [Elektronische Daten] [Takaaki Ito, Hitoshi Kitamura, Yoshiaki Inayama, Akinori Nozawa, Masayoshi Kanisawa] Summary: Cationic ferritin was used as a marker to reveal the processes of endocytosis and intracellular transport in bronchiolar and alveolar epithelia. The marker was injected into the lung via the trachea, and ultrastructural observation of the distribution of ferritin particles in bronchiolar and alveolar epithelial cells was carried out at intervals of 5, 15, 30 and 60 min after the injection. The luminal surface of the airway and the alveolar epithelium showed diffuse labeling with cationic ferritin. In general, ferritin particles were observed in vesicles and vacuoles of the bronchiolar and alveolar epithelial cells within 5 min of injection; they appeared in multivesicular bodies within 15 min. Multivesicular bodies and secondary lysosomes containing ferritin particles, some of which showed a positive reaction for acid phosphatase, were seen in the basal cytoplasm within 30 min; ferritin particles appeared in the basal lamina below the Clara cells, ciliated cells and type 2 alveolar cells within 30 min. Ferritin particles were seen in ovoid granules of some Clara cells and in lamellar inclusion bodies of many type 2 alveolar cells. Brush cells and type 1 alveolar cells took up only a small quantity of ferritin particles. Springer-Verlag, 1992 Lung nationallicence Bronchiole nationallicence Alveole nationallicence Endocytosis nationallicence Ferritin nationallicence Transmission electron microscopy nationallicence Rat (Sprague-Dawley) nationallicence Ito Takaaki Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Kitamura Hitoshi Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Inayama Yoshiaki Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Nozawa Akinori Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Kanisawa Masayoshi Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 335-340 0302-766X 268:2<335 1992 268 441 https://doi.org/10.1007/BF00318802 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318802 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Takaaki Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 700 1- Kitamura Hitoshi Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 700 1- Inayama Yoshiaki Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 700 1- Nozawa Akinori Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 700 1- Kanisawa Masayoshi Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 335-340 0302-766X 268:2<335 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer