caa a22 4500 469082240 CHVBK 20180323132953.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00318796 doi (NATIONALLICENCE)springer-10.1007/BF00318796 Immunolocalization of 67 kDa elastin-binding protein in perinatal rat lungs [Elektronische Daten] [Kojiro Wasano, Yasuhiro Hirakawa, Keiichiro Nakamura] Summary: 67 kDa elastin-binding protein (RL-67EBP) has been isolated from neonatal rat lungs by the use of an elastin-coupled affinity column, followed by elution with either lactose or synthetic elastin hexapeptide (VGVAPG), and immunohistochemistry has been used on perinatal rat lungs to determine the tissue localization of this protein. No immunoreactive structures occur in fetal lungs, or in the lungs of day-1 and-4 neonates. On day-7 after birth, immunoreactive cells appear in the subepithelial connective tissue of the intrapulmonary airways, from day-10 on, these cells become evenly distributed in the alveolar parenchyma. Occasionally, some cells occur in the alveolar air space, being free from the surface of the alveolar septum. Unpermeabilized cells obtained by bronchoalveolar lavage, show cell surface immunoreactivity, indicating that RL-67EBP is expressed on the surface membrane of the cells. From these findings, it is suggested that the immunoreactive cells are blood-borne monocytes, and that RL-67EBP may function as an elastin peptide receptor by which monocytes mobilize through interstitial connective tissue during their migration from blood to alveolar air space, where they eventually differentiate into alveolar macrophages. Springer-Verlag, 1992 Lung nationallicence Elastin-binding protein nationallicence Lectins nationallicence Galactose nationallicence Monocytes nationallicence Immunocytochemistry nationallicence Rat (Wistar) nationallicence Wasano Kojiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Hirakawa Yasuhiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Nakamura Keiichiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 277-281 0302-766X 268:2<277 1992 268 441 https://doi.org/10.1007/BF00318796 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318796 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wasano Kojiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hirakawa Yasuhiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nakamura Keiichiro Department of Anatomy, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/2(1992-05-01), 277-281 0302-766X 268:2<277 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer