caa a22 4500 469082267 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318694 doi (NATIONALLICENCE)springer-10.1007/BF00318694 An identified dorsal unpaired median neurone and bilaterally projecting neurones exhibiting bovine pancreactic polypeptide-like/FMRFamide-like immunoreactivity in abdominal ganglia of the migratory locust [Elektronische Daten] [Michael Ferber, H. Pflüger] Summary: Three antisera were used to study the distribution and anatomy of bovine pancreatic polypeptide (BPP)-like/FMRFamide-like immunoreactive neurones within the unfused abdominal ganglia of the migratory locust, Locusta migratoria. All the antisera used stained two or more clusters of perikarya, localized anteriorly and posteriorly near the midline within each unfused abdominal ganglion. Double labelling experiments with intracellular dye injection, or differential backfilling, combined with subsequent immunostaining were carried out to identify these neurones. Two of the antisera (antisera 1 and 2, both raised against FMRFamide) stained three groups of midline neurones, located anterior dorsal, anterior ventral and posterior dorsal within the ganglion. Neurones of the former of these two clusters projected via the anterior median nerve to a neurohaemal organ. The posterior cluster of midline cells comprised immunopositive perikarya all but one of which also projected via the anterior median nerve to innervate the neurohaemal organ. Double labelling with Lucifer yellow and antisera 1 and 2 showed that the remaining neurone was the previously identified doral unpaired median (DUM)heart1 neurone. The third antiserum (AK141), also raised against FMRFamide, stained neurones within an anterior dorsal cluster, and in a posterior cluster. Double labelling with differential Co2+/Ni2+-backfilling and the antiserum 3 (AK141) demonstrated that the large neurones of both clusters belonged to the population of bilaterally projecting neurones (BPNs), including the DUMheart1 neurone. Since the antisera cross-react with BPP and fail to label neurones when preadsorped with BPP or FMRFamide, we conclude that the labelled neurones contain polypeptides of the FMRFamide/BPP-family. Springer-Verlag, 1992 Ganglia, invertebrate nationallicence Neurosecretory cells nationallicence DUM neurone nationallicence Neurohemal organs nationallicence Heart nationallicence Immunocytochemistry nationallicence Locusta migratoria (Insecta) nationallicence Ferber Michael Fakultät für Biologie, Universität Konstanz, Postfach 5560, W-7750, Konstanz 1, Federal Republic of Germany aut Pflüger H. Institut für Neurobiologie, Freie Universität Berlin, Königin-Luise-Strasse 28-30, W-1000, Berlin 33, Federal Republic of Germany aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 85-98 0302-766X 267:1<85 1992 267 441 https://doi.org/10.1007/BF00318694 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318694 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferber Michael Fakultät für Biologie, Universität Konstanz, Postfach 5560, W-7750, Konstanz 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Pflüger H. Institut für Neurobiologie, Freie Universität Berlin, Königin-Luise-Strasse 28-30, W-1000, Berlin 33, Federal Republic of Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 85-98 0302-766X 267:1<85 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer