caa a22 4500 469082291 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318685 doi (NATIONALLICENCE)springer-10.1007/BF00318685 Immunohistochemical localization of Na+-dependent glucose transporter in rat jejunum [Elektronische Daten] [Kuniaki Takata, Toshiko Kasahara, Michihiro Kasahara, Osamu Ezaki, Hiroshi Hirano] Summary: Glucose is actively absorbed via a Na+-dependent active glucose transporter (Na-GT) in the small intestine. We raised a polyclonal antibody against the peptide corresponding to amino acids 564-575 of rabbit intestinal Na-GT, and localized it immunohistochemically in the rat jejunum. By means of immunofluorescence staining, Na-GT was located at the brush border of the absorptive epithelial cells of the intestinal villi. Electron-microscopic examination showed that Na-GT was localized at the plasma membrane of the apical microvilli of these cells. Little Na-GT was found at the basolateral plasma membrane. Along the crypt-villus axis, all of the absorptive epithelial cells in the villus were positive for Na-GT. In addition to the brush border staining, the supranuclear positive staining, which was shown to be the Golgi apparatus by use of electron microscopy, was seen in cells located between the base to the middle of the villus. Cells in crypts exhibited little or no staining for Na-GT. Goblet cells scattered in the intestinal epithelium were negative for Na-GT staining. These observations show that Na-GT is specific to the apical plasma membrane of the absorptive epithelial cells, and that the onset of Na-GT synthesis may occur near the crypt-villus junction. Springer-Verlag, 1992 Jejunum nationallicence Na+-dependent active glucose transporter nationallicence Absorptive epithelial cell nationallicence Crypt-villus axis nationallicence Plasma membrane nationallicence Golgi apparatus nationallicence Rat (Sprague-Dawley) nationallicence Takata Kuniaki Department of Anatomy, Kyorin University School of Medicine, Shinkawa, 181, Mitaka, Tokyo, Japan aut Kasahara Toshiko Laboratory of Biophysics, School of Medicine, Teikyo University, 192-03, Hachioji, Tokyo, Japan aut Kasahara Michihiro Laboratory of Biophysics, School of Medicine, Teikyo University, 192-03, Hachioji, Tokyo, Japan aut Ezaki Osamu Division of Clinical Nutrition, National Institute of Health and Nutrition, 162, Toyama, Tokyo, Japan aut Hirano Hiroshi Department of Anatomy, Kyorin University School of Medicine, Shinkawa, 181, Mitaka, Tokyo, Japan aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 3-9 0302-766X 267:1<3 1992 267 441 https://doi.org/10.1007/BF00318685 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318685 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takata Kuniaki Department of Anatomy, Kyorin University School of Medicine, Shinkawa, 181, Mitaka, Tokyo, Japan aut NATIONALLICENCE 700 1- Kasahara Toshiko Laboratory of Biophysics, School of Medicine, Teikyo University, 192-03, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Kasahara Michihiro Laboratory of Biophysics, School of Medicine, Teikyo University, 192-03, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Ezaki Osamu Division of Clinical Nutrition, National Institute of Health and Nutrition, 162, Toyama, Tokyo, Japan aut NATIONALLICENCE 700 1- Hirano Hiroshi Department of Anatomy, Kyorin University School of Medicine, Shinkawa, 181, Mitaka, Tokyo, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 3-9 0302-766X 267:1<3 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer