caa a22 4500 469082305 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318703 doi (NATIONALLICENCE)springer-10.1007/BF00318703 Local production of fibronectin by ectopic human retinal cells [Elektronische Daten] [P. Hiscott, H. Waller, I. Grierson, M. Butler, D. Scott] Summary: The distribution of fibronectin mRNA and fibronectin in adult human retina and epiretinal membranes was investigated by in situ hybridisation and immunohistochemical techniques. The cells in normal adult retina contained little or no fibronectin mRNA and the retina only showed fibronectin immunoreactivity in retinal vessels. The cells in detached neuroretina did not contain fibronectin message but the vitreoretinal interface of the detached retina exhibited variable fibronectin immunoreactivity. Retinal glia, retinal pigment epithelium and fibroblast-like cells in membranes at the vitreoretinal juncture (epiretinal membranes) showed variable labelling with the fibronectin mRNA probe and all the membranes immunostained for fibronectin. No difference could be detected between membrane cell types in the intensity of labelling with the mRNA probe or for fibronectin immunoreactivity. The results indicate that cells in situ in attached and detached adult human retina do not produce fibronectin. Although fibronectin at the vitreoretinal juncture in retinal detachment is probably partly derived from plasma fibronectin resulting from breakdown of the blood-retinal barrier, ectopic retinal cells produce fibronectin and contribute to the glycoprotein in epiretinal membranes. Springer-Verlag, 1992 Retina nationallicence Proliferative vitreoretinopathy nationallicence Epiretinal membrane nationallicence Fibronectin nationallicence In situ hybridisation nationallicence Immunohistochemistry nationallicence Man nationallicence Hiscott P. Department of Pathology, Institute of Ophthalmology, 17-25 Cayton Street, EC1V 9AT, London, UK aut Waller H. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut Grierson I. Department of Clinical Science, Institute of Ophthalmology, 17-25 Cayton Street, EC1V 9AT, London, UK aut Butler M. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut Scott D. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 185-192 0302-766X 267:1<185 1992 267 441 https://doi.org/10.1007/BF00318703 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318703 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiscott P. Department of Pathology, Institute of Ophthalmology, 17-25 Cayton Street, EC1V 9AT, London, UK aut NATIONALLICENCE 700 1- Waller H. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut NATIONALLICENCE 700 1- Grierson I. Department of Clinical Science, Institute of Ophthalmology, 17-25 Cayton Street, EC1V 9AT, London, UK aut NATIONALLICENCE 700 1- Butler M. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut NATIONALLICENCE 700 1- Scott D. Department of Immunology and Rheumatology, St. Bartholomew's Hospital, 17-25 Cayton Street, EC1V 9AT, London, UK aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 185-192 0302-766X 267:1<185 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer