caa a22 4500 469082313 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318695 doi (NATIONALLICENCE)springer-10.1007/BF00318695 Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes [Elektronische Daten] [Miranda Grounds, Kerryn Garrett, May Lai, Woodring Wright, Manfred Beilharz] Summary: The activation of mononuclear muscle precursor cells after crush injury to mouse tibialis anterior muscles was monitored in vivo by in situ hybridization with MyoD1 and myogenin probes. These genes are early markers of skeletal muscle differentiation and have been extensively studied in vitro. The role in vivo of these regulatory proteins during myogenesis of mature muscle has not been studied previously. MyoD1 and myogenin mRNA were present in occasional mononuclear cells of uninjured muscle. Increased MyoD1 and myogenin mRNA sequences in mononuclear cells were detected as early as 6 h after injury, peaked between 24 and 48 h, and thereafter declined to pre-injury levels at about 8 days. The mRNAs were detected in mononuclear cells throughout the muscle, with the majority of cells located some distance from the site of crush injury. The presence of MyoD1 and myogenin mRNA at 6 to 48 h indicates that transcription of these genes is occurring at the same time as replication of muscle precursor cells in vivo. At no time were significant levels of mRNA for these genes detected in myotubes. MyoD1 and myogenin provide precise markers for the very early identification and study of mononuclear skeletal muscle precusor cells in muscle regenerating in vivo. Springer-Verlag, 1992 Skeletal muscle nationallicence Myogenesis nationallicence Muscle regeneration nationallicence MyoD1 nationallicence Myogenin nationallicence In situ hybridisation nationallicence Mouse (Swiss SJL/J) nationallicence Grounds Miranda Department of Pathology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut Garrett Kerryn Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut Lai May Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut Wright Woodring Department of Cell Biology and Anatomy, The University of Texas Southwestern Medical Center at Dallas, 75235, Dallas, TX, USA aut Beilharz Manfred Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 99-104 0302-766X 267:1<99 1992 267 441 https://doi.org/10.1007/BF00318695 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318695 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grounds Miranda Department of Pathology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut NATIONALLICENCE 700 1- Garrett Kerryn Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut NATIONALLICENCE 700 1- Lai May Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut NATIONALLICENCE 700 1- Wright Woodring Department of Cell Biology and Anatomy, The University of Texas Southwestern Medical Center at Dallas, 75235, Dallas, TX, USA aut NATIONALLICENCE 700 1- Beilharz Manfred Department of Microbiology, Queen Elizabeth II Medical Centre, The University of Western Australia, 6009, Nedlands, Western Australia, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 99-104 0302-766X 267:1<99 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer