caa a22 4500 469082380 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318700 doi (NATIONALLICENCE)springer-10.1007/BF00318700 Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man [Elektronische Daten] [J. van Deijnen, C. Hulstaert, G. Wolters, R. van Schilfgaarde] Summary: The presence and distribution in the peri-insular region of extracellular matrix, and in particular basement membrane, was investigated in a comparative study comprising pancreata of rat, dog, pig, and man. Basement membrane markers, collagen type-IV and laminin, were determined immunohistochemically. Additional information pertaining to the structural relationships between endocrine and exocrine pancreas, in particular cell-to-cell and cell-to-matrix contacts, was obtained by electron microscopy. In pig, very little periinsular capsule is present, and the structural integration of the porcine islet in the exocrine pancreas almost exclusively depends on cell-to-cell adhesion. In the canine pancreas, the islets are almost completely encapsulated with very little direct exocrine-to-endocrine cell-to-cell contact. In rat and man, the situation is intermediate with a tendency towards predominance of cell-to-matrix adhesion. The intra-insular adhesion mechanisms depend largely on cell-to-cell adhesion in all four species. The ultrastructural results suggest that collagenase preparations employed in islet isolation procedures should be of high purity as to preserve the protease-sensitive intra-islet cell-to-cell adhesion. Under these conditions, however, the endocrine-to-exocrine cell-to-cell contacts will be conserved also, resulting in an exocrine-tissue contamination of the islets of Langerhans. Consequently, additional steps for the effective removal of exocrine tissue and the purification of islets are required. Springer-Verlag, 1992 Pancreatic islet isolation nationallicence Peri-insular extracellular matrix nationallicence Collagen type-IV nationallicence Laminin nationallicence Pancreas dissociation nationallicence Rat (Wistar) nationallicence Dog nationallicence Man nationallicence van Deijnen J. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut Hulstaert C. Department of Histology and Cell Biology, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut Wolters G. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut van Schilfgaarde R. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 139-146 0302-766X 267:1<139 1992 267 441 https://doi.org/10.1007/BF00318700 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318700 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Deijnen J. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Hulstaert C. Department of Histology and Cell Biology, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Wolters G. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut NATIONALLICENCE 700 1- van Schilfgaarde R. Department of Surgery, University of Groningen, Bloemsingel 1, NL-9713 BZ, Groningen, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 139-146 0302-766X 267:1<139 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer