caa a22 4500 469082410 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00318702 doi (NATIONALLICENCE)springer-10.1007/BF00318702 Differentiation of the melanotrophic cells of rat pituitary primordium in organotypic culture in defined medium [Elektronische Daten] [P. Vuillez, F. René, M. Plante, C. Hindelang, M. Klein, J. Félix, M. Stoeckel] Summary: Organotypic cultures, in defined medium, of pituitary primordia obtained from 15-day-old rat fetuses were performed in order to study the in vitro differentiation of melanotrophic cells. The morphological and ultrastructural features of the transplants resembled those of the gland developing in vivo. In situ hybridization on semi-thin sections, using a 35S-labelled oligonucleotide probe, revealed pro-opiomelanocortin-mRNA-containing cells on the first day of culture in the anterior lobe and after 2-3 days in the intermediate lobe. Immunoperoxidase labelling of adjacent sections showed that the same cells reacted with antibodies against α-melanocyte-stimulating hormone (αMSH), γ3 and adrenocorticotropic hormone in both lobes. The pro-opiomelanocortin-mRNA-containing cells formed progressively conspicuous areas in the intermediate lobe, which was almost uniformly labelled after 6 days. In the anterior lobe, these cells remained scattered in small cell groups, and colloidal gold immunolabelling showed the progressive disappearance of αMSH labelling from the secretory vesicles in cells exhibiting morphological features of adult corticotrophic cells. Both the αMSH content of the explants and αMSH release into the culture medium increased with time. Treatment with the dopamine agonist bromocriptine induced a strong dose-dependent decrease in αMSH secretion, which was significant after 3 days in culture, indicating that dopamine D2 receptors are able to regulate hormonal release of melanotrophic cells at early stages. This system constitutes a suitable model for further studies of factors controlling cell differentiation and cellular interactions involved in histogenesis. Springer-Verlag, 1992 Fetal intermediate lobe nationallicence Tissue culture nationallicence Immunocytochemistry nationallicence In situ hybridization nationallicence Dopamine nationallicence Rat (Wistar) nationallicence Vuillez P. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut René F. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Plante M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Hindelang C. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Klein M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Félix J. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Stoeckel M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 169-183 0302-766X 267:1<169 1992 267 441 https://doi.org/10.1007/BF00318702 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318702 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vuillez P. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- René F. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- Plante M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- Hindelang C. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- Klein M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- Félix J. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 700 1- Stoeckel M. Laboratoire de Physiologie, URA CNRS no 1446, 21, Rue Descartes, F-67084, Strasbourg, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/1(1992-01-01), 169-183 0302-766X 267:1<169 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer