caa a22 4500 469082518 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319372 doi (NATIONALLICENCE)springer-10.1007/BF00319372 Characterization and fine-structural localization of actin-and fibronectin-like proteins in planaria ( Dugesia lugubris s.l. ) [Elektronische Daten] [Rita Pascolini, Fausto Panara, Ines Di Rosa, Anna Fagotti, Sergio Lorvik] Summary: Actin- and fibronectin-like proteins were characterized in the planarian, Dugesia lugubris s.l., by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting analysis using antisera to vertebrate actin and fibronectin. These antisera recognized protein bands of 42 kDa and 220 kDa, respectively. In addition, the immunohistochemical distribution of both actin- and fibronectin-like material was examined by using immuno-electron microscopy. Actin-like protein was localized in myofibrils in various differentiation stages, and in the peripheral cytoplasm and lamellipodia of cells that were migrating. The fibronectin-like component was associated with the extracellular matrix in the fibrillar structures and with the surface of the migrating cells. Our data suggest that similar cellular and molecular mechanisms are involved in cell-matrix interactions and in the morphogenesis of living organisms at different evolutionary levels. Springer-Verlag, 1992 Actin-like protein nationallicence Fibronectin-like protein nationallicence Regeneration nationallicence Cell migration nationallicence Immunocytochemistry nationallicence Dugesia lugubris (Tricladida) nationallicence Pascolini Rita Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut Panara Fausto Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut Di Rosa Ines Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut Fagotti Anna Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut Lorvik Sergio Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 499-506 0302-766X 267:3<499 1992 267 441 https://doi.org/10.1007/BF00319372 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319372 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pascolini Rita Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Panara Fausto Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Di Rosa Ines Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Fagotti Anna Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Lorvik Sergio Institute of Comparative Anatomy, University of Perugia, Via Pascoli, I-06100, Perugia, Italy aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 499-506 0302-766X 267:3<499 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer