caa a22 4500 469082542 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319377 doi (NATIONALLICENCE)springer-10.1007/BF00319377 In situ hybridization of corticotropin-releasing factor-encoding messenger RNA in the hypothalamus of the white sucker, Catostomus commersoni [Elektronische Daten] [Yuji Okawara, David Ko, Steven Morley, Dietmar Richter, Karl Lederis] Summary: In situ hybridization procedure with a 32P-labelled synthetic oligonucleotide probe was used to detect corticotropin-releasing factor-encoding messenger RNA (CRF mRNA) in the hypothalamus of the white sucker, Catostomus commersoni. Adjacent sections were immunostained by a sucker CRF-specific antiserum. CRF mRNA-containing neurons were identified by autoradiography in the magnocellular and parvocellular subdivisions of the preoptic nucleus (PON). Many of these neurons were also immunostained by sucker antiserum, showing the same distribution patterns. These results confirm the presence of CRF mRNA and CRF peptide in the white sucker hypothalamus and support the view that the magnocellular and parvocellular neurons of the PON may be involved in the control of adrenocorticotropic hormone secretion from the pituitary in the white sucker. Springer-Verlag, 1992 In situ hybridization nationallicence Immunohistochemistry nationallicence Corticotropin-releasing factor nationallicence Messenger RNA nationallicence Preoptic nucleus nationallicence Catostomus commersoni (Teleostei) nationallicence Okawara Yuji Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada aut Ko David Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada aut Morley Steven Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, W-2000, Hamburg, Federal Republic of Germany aut Richter Dietmar Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, W-2000, Hamburg, Federal Republic of Germany aut Lederis Karl Department of Pharmacology, University of Calgary, 147 Carthew Street, V9N 5C9, Comox, B.C., Canada aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 545-549 0302-766X 267:3<545 1992 267 441 https://doi.org/10.1007/BF00319377 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319377 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okawara Yuji Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada aut NATIONALLICENCE 700 1- Ko David Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada aut NATIONALLICENCE 700 1- Morley Steven Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, W-2000, Hamburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Richter Dietmar Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, W-2000, Hamburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lederis Karl Department of Pharmacology, University of Calgary, 147 Carthew Street, V9N 5C9, Comox, B.C., Canada aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 545-549 0302-766X 267:3<545 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer