caa a22 4500 469082550 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319365 doi (NATIONALLICENCE)springer-10.1007/BF00319365 Heterogeneous distribution of the precursor of type I and type III collagen and fibronectin in the rough endoplasmic reticulum of palatal mesenchymal cells of the mouse embryo cultured in ascorbate-depleted medium [Elektronische Daten] [Kojiro Kurisu, Yasuyoshi Ohsaki, Kengo Nagata, Tetsuichiro Inai, Toshio Kukita] Summary: In order to examine the intracellular distribution of precursors of type I and type III collagen and fibronectin in the palatal mesenchymal (MEPM) cells of the mouse embryo cultured under ascorbate-deficient conditions, immuno-electron-microscopic studies were carried out by use of affinity purified antibodies for these proteins. MEPM cells were obtained from the palatal shelves of 14-day-old mouse fetuses and cultured for 3-7 days in medium, either with or without 50 ng/dish/day ascorbic acid. Results obtained were as follows: (1) Although the rough endoplasmic reticulum (rER) of MEPM cells cultured for 5 days in ascorbate-supplemented medium was flattened, that in cells cultured in ascorbate-deficient medium had a distended or vesicular appearance. (2) Vesicular or distended rER showed heterogeneous staining for both type I and type III collagen, namely, some parts of rER showed positive staining for both types of collagen, while others showed negative staining. (3) Both type I and type III collagen showed codistribution in the same vesicular rER. (4) Vesicular rER showed negative or very faint labelling for fibronectin. These results may suggest regional differences in the function of rER. Springer-Verlag, 1992 Collagens nationallicence Rough endoplasmic reticulum nationallicence Palate nationallicence Mesenchymal cell nationallicence Mouse (CF1) nationallicence Kurisu Kojiro Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Ohsaki Yasuyoshi Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Nagata Kengo Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Inai Tetsuichiro Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Kukita Toshio Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 429-435 0302-766X 267:3<429 1992 267 441 https://doi.org/10.1007/BF00319365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319365 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurisu Kojiro Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ohsaki Yasuyoshi Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nagata Kengo Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Inai Tetsuichiro Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kukita Toshio Second Department of Anatomy, Faculty of Dentistry, Kyushu University 61, Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 429-435 0302-766X 267:3<429 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer