caa a22 4500 469082569 CHVBK 20180323132954.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319378 doi (NATIONALLICENCE)springer-10.1007/BF00319378 The VD1/RPD2 neuronal system in the central nervous system of the pond snail Lymnaea stagnalis studied by in situ hybridization and immunocytochemistry [Elektronische Daten] [R. Kerkhoven, R. Croll, M. Ramkema, J. Van Minnen, J. Bogerd, H. Boer] Summary: VD1 and RPD2 are two giant neuropeptidergic neurons in the central nervous system (CNS) of the pond snail Lymnaea stagnalis. We wished to determine whether other central neurons in the CNS of L. stagnalis express the VD1/RPD2 gene. To this end, in situ hybridization with the cDNA probe of the VD1/RPD2 gene and immunocytochemistry with antisera specific to VD1 and RPD2 (the α1-antiserum, Mab4H5 and ALMA 6) and to R15 (the α1 and 16-mer antisera) were performed on alternate tissue sections. A VD1/RPD2 neuronal system comprising three classes of neurons (A1-A3) was found. All neurons of the system express the gene. Division into classes is based on immunocytochemical characteristics. Class A1 neurons (VD1 and RPD2) immunoreact with the α1-antiserum, Mab4H5 and ALMA 6. Class A2 neurons (1-5 small and 1-5 medium sized neurons in the visceral and right parietal ganglion, and two clusters of small neurons and 5 medium-sized neurons in the cerebral ganglia) immunoreact with the α1-antiserum and Mab4H5, but not with ALMA 6. Class A3 neurons (3-4 medium-sized neurons and a cluster of 4-5 small neurons located in the pedal ganglion) immunoreact with the α1-antiserum only. All neurons of the system are immunonegative to the R15 antisera. The observations suggest that the neurons of the VD1/RPD2 system produce different sets of neuropeptides. A group of approximately 15 neurons (class B), scattered in the ganglia, immunostained with one or more of the antisera, but did not react with the cDNA probe in in situ hybridization. Springer-Verlag, 1992 Nervous system, central nationallicence VD1/RPD2 system nationallicence Neuropeptide immunocytochemistry nationallicence Hybridization, in situ nationallicence Immunocytochemistry nationallicence Lymnaea stagnalis (Mollusca) nationallicence Kerkhoven R. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Croll R. Department of Physiology and Biophysics, Dalhousie University, Halifax, Canada aut Ramkema M. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Van Minnen J. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Bogerd J. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Boer H. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 551-559 0302-766X 267:3<551 1992 267 441 https://doi.org/10.1007/BF00319378 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319378 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kerkhoven R. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Croll R. Department of Physiology and Biophysics, Dalhousie University, Halifax, Canada aut NATIONALLICENCE 700 1- Ramkema M. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Van Minnen J. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Bogerd J. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Boer H. Department of Organismic Zoology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 551-559 0302-766X 267:3<551 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer