caa a22 4500 469082607 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319364 doi (NATIONALLICENCE)springer-10.1007/BF00319364 Time-related changes in the labeling pattern of motor and sensory neurons innervating the gastrocnemius muscle, as revealed by the retrograde transport of the cholera toxin B subunit [Elektronische Daten] [M. Hirakawa, J. McCabe, M. Kawata] Summary: Morphological changes in the motor and sensory neurons in the lumbar spinal cord and the dorsal root ganglia were investigated at different survival times following the injection of the B subunit of cholera toxin (CTB) into the medial gastrocnemius muscle. Unconjugated CTB, visualized immunohistochemically, was found to be retrogradely transported through ventral and dorsal roots to motor neurons in the anterior horn, each lamina in the posterior horn, and ganglion cells in the dorsal root ganglia at L3-L6. The largest numbers of labeled motor neurons and ganglion cells were observed 72 h after the injection of CTB. Thereafter, labeled ganglion cells were significantly decreased in number, whereas the amount of labeled motor neurons showed a slight reduction. Motor neurons had extensive dendritic trees filled with CTB, reaching lamina VII and even the pia mater of the lateral funiculus. Labeling was also seen in the posterior horn, but the central and medial parts of laminae II and III had the most extensively labeled varicose fibers, the origin of which was the dorsal root ganglion cells. The results indicate that CTB is taken up by nerve terminals and can serve as a sensitive retrogradely transported marker for identifying neurons that innervate a specific muscle. Springer-Verlag, 1992 Cholera toxin B subunit nationallicence Motoneurons nationallicence Primary afferent neurons nationallicence Spinal cord nationallicence Retrograde transport nationallicence Immunohistochemistry nationallicence Rat (Wistar) nationallicence Hirakawa M. Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, 602, Kyoto, Japan aut McCabe J. Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, 20889-4799, Bethesda, MD, USA aut Kawata M. Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, 602, Kyoto, Japan aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 419-427 0302-766X 267:3<419 1992 267 441 https://doi.org/10.1007/BF00319364 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319364 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirakawa M. Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, 602, Kyoto, Japan aut NATIONALLICENCE 700 1- McCabe J. Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, 20889-4799, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Kawata M. Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, 602, Kyoto, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 419-427 0302-766X 267:3<419 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer