caa a22 4500 469082631 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00319369 doi (NATIONALLICENCE)springer-10.1007/BF00319369 Light- and electron-microscopic immunocytochemistry of a molluscan insulin-related peptide in the central nervous system of Planorbarius corneus [Elektronische Daten] [D. Sonetti, W. van Heumen, E. Roubos] Summary: Two groups of cerebral dorsal cells of the pulmonate snail Planorbarius corneus stain positively with antisera raised against synthetic fragments of the B- and C-chain of the molluscan pro-insulin-related prohormone, proMIP-I, of another pulmonate snail, Lymnaea stagnalis. At the light-microscopic level the somata of the dorsal cells and their axons and neurohemal axon terminals in the periphery of the paired median lip nerves are immunoreactive with both antisera. Furthermore, the canopy cells in the lateral lobes of the cerebral ganglia are positive. In addition, MIPB-immunoreactive neurons are found in most other ganglia of the central nervous system. At the ultrastructural level, pale and dark secretory granules are found in somata and axon terminals of the dorsal cells. Dark granules are about 4 times as immunoreactive to both antisera as pale granules. Release of anti-MIPB- and anti-MIPC-immunopositive contents of the secretory granules by exocytosis is apparent in material treated according to the tannic acid method. It is concluded that the dorsal and canopy cells synthesize a molluscan insulin-related peptide that is packed in the cell body into secretory granules and that is subsequently transported to the neurohemal axon terminals and released into the hemolymph by exocytosis. Thus, MIP seems to act as a neurohormone on peripheral targets. On the basis of the analogy between the dorsal cells and the MIP-producing cells in L. stagnalis, it is proposed that the dorsal cells of P. corneus are involved in the control of body growth and associated processes. Springer-Verlag, 1992 Cerebral ganglia nationallicence Neurohormones nationallicence Molluscan insulin-related peptide nationallicence Immunocytochemistry nationallicence Tannic acid nationallicence Planorbarius corneus (Mollusca) nationallicence Sonetti D. Dipartimento di Biologia Animale, Università di Modena, Via Berengario 14, I-41100, Modena, Italy aut van Heumen W. Department of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Roubos E. Department of Animal Physiology, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands aut Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 473-481 0302-766X 267:3<473 1992 267 441 https://doi.org/10.1007/BF00319369 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319369 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sonetti D. Dipartimento di Biologia Animale, Università di Modena, Via Berengario 14, I-41100, Modena, Italy aut NATIONALLICENCE 700 1- van Heumen W. Department of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Roubos E. Department of Animal Physiology, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/3(1992-03-01), 473-481 0302-766X 267:3<473 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer