caa a22 4500 469082739 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302956 doi (NATIONALLICENCE)springer-10.1007/BF00302956 Appearance of an intra-acrosomal antigen during the terminal step of spermiogenesis in the rat [Elektronische Daten] [I. Tanii, K. Toshimori, S. Araki, C. Ōura] Summary: In a survey of sperm antigens in the rat, a new intra-acrosomal antigen was found using a monoclonal antibody MC41 raised against rat epididymal spermatozoa. The MC41 was immunoglobulin G1 and recognized spermatozoa from rat, mouse and hamster. Indirect immunofluorescence with MC41 specifically stained the crescent region of the anterior acrosome of the sperm head. Immuno-gold electron microscopy demonstrated that the antigen was localized within the acrosomal matrix. Immunoblot study showed that MC41 recognized a band of approximately 165000 dalton in the extract of rat sperm from the cauda epididymidis. Immunohistochemistry with MC41 demonstrated that the antigen was first detected in approximately step-2 spermatids, and distributed over the entire cytoplasmic region of spermatids from step 2 to early step 19. The head region became strongly stained in late step-19 spermatids and then in mature spermatozoa. Distinct immunostaining was not found in the developing acrosome of spermatids throughout spermiogenesis. These results suggest that the MC41 antigen is a unique intra-acrosomal antigen which is accumulated into the acrosome during the terminal step of spermiogenesis. Springer-Verlag, 1992 Intra-acrosomal protein nationallicence Monoclonal antibody nationallicence Acrosome development nationallicence Spermiogenesis nationallicence Intracellular transport nationallicence Testis nationallicence Rat (Wistar) nationallicence Mouse (ICR, BALB/c) nationallicence Tanii I. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut Toshimori K. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut Araki S. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut Ōura C. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 203-208 0302-766X 267:2<203 1992 267 441 https://doi.org/10.1007/BF00302956 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302956 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanii I. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Toshimori K. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Araki S. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Ōura C. Department of Anatomy, Miyazaki Medical College, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 203-208 0302-766X 267:2<203 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer