caa a22 4500 469082755 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302965 doi (NATIONALLICENCE)springer-10.1007/BF00302965 Functional implications of the projections of neurons from individual labellar sensillum of Drosophila melanogaster as revealed by neuronal-marker horseradish peroxidase [Elektronische Daten] [Shubha Shanbhag, R. Naresh Singh] Summary: Earlier studies using Golgi silver impregnations from the labellar sensilla of adult Drosophila melanogaster revealed seven types of sensory axons projecting into the suboesophageal ganglion of the brain. These sensory terminals were designated as coiled fibres (type-I), shrubby fibres (type-II), ipsilateral ventral fibres (type-III), ipsilateral dorsal fibres (type-IV), contralateral ventral fibres (type-V), contralateral dorsal fibres (type-VI), and central fibres (type-VII). The present study identifies the projections of sensory neurons present in a single labellar taste-sensillum, using the neuronal marker horseradish peroxidase (HRP). Although the taste sensillum in question has five neurons, in a given experiment only one or at the most two neurons are labelled. The type of neuron labelled was usually specific to the stimulant solute (sucrose, sodium chloride or potassium chloride) present in the HRP solution. Although type-II fibres get labelled most of the time, irrespective of the stimulant present in HRP solution, type-IV fibres are labelled when attractants (0.1 M sucrose or ≤0.1 M sodium chloride) are used as stimulants in HRP solution. Type-VI fibres are labelled when the stimulant is 0.1 M potassium chloride, a repellent. HRP dissolved in distilled water revealed type-I coiled fibres. Besides revealing projections of sensillar neurons to the brain the present technique also inferred their possible function. Incubation of whole-brain tissue with 0.04% 3,3′-diaminobenzidine tetrahydrochloride in presence of 0.06% hydrogen peroxide suggested that the glomerular organization is also present in the taste-sensory region as it is in olfactory neuropile. Springer-Verlag, 1992 Labellar sensillum nationallicence Projections nationallicence Functional implications nationallicence Horseradish-peroxidase uptake nationallicence Drosophila melanogaster (Insecta) nationallicence Shanbhag Shubha Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Navt Nagar, Colaba, 400 005, Bombay, India aut Naresh Singh R. Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Navt Nagar, Colaba, 400 005, Bombay, India aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 273-282 0302-766X 267:2<273 1992 267 441 https://doi.org/10.1007/BF00302965 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302965 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shanbhag Shubha Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Navt Nagar, Colaba, 400 005, Bombay, India aut NATIONALLICENCE 700 1- Naresh Singh R. Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Navt Nagar, Colaba, 400 005, Bombay, India aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 273-282 0302-766X 267:2<273 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer