caa a22 4500 46908278X CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302968 doi (NATIONALLICENCE)springer-10.1007/BF00302968 Localization of luteinizing hormone β-mRNA by in situ hybridization in the sheep pars tuberalis [Elektronische Daten] [J. Pelletier, R. Counis, M.-M. de Reviers, Y. Tillet] Summary: The localization of luteinizing hormone beta (LHβ)-mRNA was studied by in situ hybridization in the pars tuberalis of sheep using a homologous sheep double-stranded 32P-or 35S-cDNA. The labelled cDNA probe detected one mRNA sequence in the pars tuberalis by Northern blot analysis; this sequence was similar to that detected in the pituitary. In situ, the labelling of LHβ-mRNA in the horizontal and sagittal tissue sections was found throughout the pars tuberalis. This labelling was prevented by adding an excess of cold probe or treating the sections by ribonuclease before in situ hybridization. Controls showed a labelling in the pars distalis, but not in the median eminence, hypothalamus, cerebral cortex and liver sections. Double labelling by using a specific LHβ-antiserum indicated that the labelling of LHβ-mRNA appeared more intense in LH-containing cells that were found only in the ventral part of the pars tuberalis. These results suggest that the entire pars tuberalis is able to produce the LHβ subunit, but that the level of translation greatly varies according to the location of the cells. Springer-Verlag, 1992 Luteinizing hormone beta-messenger RNA nationallicence In situ hybridization nationallicence Immunohistochemistry nationallicence Pars tuberalis nationallicence Sheep nationallicence Pelletier J. Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut Counis R. Laboratoire des hormones polypeptidiques, CNRS, F-91198, Gif-sur-Yvette Cedex, France aut de Reviers M.-M Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut Tillet Y. Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 301-306 0302-766X 267:2<301 1992 267 441 https://doi.org/10.1007/BF00302968 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302968 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pelletier J. Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut NATIONALLICENCE 700 1- Counis R. Laboratoire des hormones polypeptidiques, CNRS, F-91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- de Reviers M.-M Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut NATIONALLICENCE 700 1- Tillet Y. Laboratoire de Physiologie de la Reproduction, INRA, F-37380, Nouzilly, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 301-306 0302-766X 267:2<301 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer