caa a22 4500 469082801 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302966 doi (NATIONALLICENCE)springer-10.1007/BF00302966 Stanniocalcin-like immunoreactivity in the corpuscles of Stannius of the bowfin Amia calva L [Elektronische Daten] [Luciano Marra, John Youson, David Butler, Henry Friesen, Graham Wagner] Summary: We used an antiserum against salmon stanniocalcin in an immunohistochemical, immunocytochemical, and Western blot analysis of bowfin (Amia calva) corpuscles of stannius. The bowfin is one of two extant holostean species with ancient ancestral links to modern-day bony fishes. The corpuscles of Stannius (white corpuscles) of the bowfin were scattered throughout much of the kidney among the adrenocortical homolog (yellow corpuscles) but could be distinguished from the adrenocortical homolog by their positive staining with both the periodic acid-Schiff reaction and a salmon stanniocalcin antiserum. Immunoreactivity was confined to cytoplamic granules and was absent when the antiserum was blocked with salmon stanniocalcin or with a crude extract of bowfin corpuscles of Stannius. When bowfin corpuscle-of-Stannius extracts were subjected to sodium dodecyl sulphate electrophoresis and Western blot analysis, two closely spaced bands were evident (43-45 kDa). Staining of both bands was abolished by pre-absorption of the antiserum with salmon stanniocalcin. In comparison to salmon stanniocalcin, the reputed bowfin hormone migrated faster in gels, suggesting a smaller apparent size. The purification of bowfin stanniocalcin should yield important new information regarding the evolution of this unique calcium-regulating hormone. Springer-Verlag, 1992 Corpuscles of Stannius nationallicence Stanniocalcin nationallicence Immunocytochemistry nationallicence Western blot nationallicence Amia calva (Holostei) nationallicence Marra Luciano Department of Anatomy, University of Toronto, M1C 1A4, Toronto, Ontario, Canada aut Youson John Department of Anatomy, University of Toronto, M1C 1A4, Toronto, Ontario, Canada aut Butler David Department of Zoology, Division of Life Sciences, University of Toronto, Scarborough Campus, M1C 1A4, Ontario, Canada aut Friesen Henry Department of Physiology, University of Manitoba, R3E 0W5, Winnipeg, Manitoba, Canada aut Wagner Graham Department of Physiology, University of Western Ontario, N6A 5C1, London, Ontario, Canada aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 283-290 0302-766X 267:2<283 1992 267 441 https://doi.org/10.1007/BF00302966 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302966 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marra Luciano Department of Anatomy, University of Toronto, M1C 1A4, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Youson John Department of Anatomy, University of Toronto, M1C 1A4, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Butler David Department of Zoology, Division of Life Sciences, University of Toronto, Scarborough Campus, M1C 1A4, Ontario, Canada aut NATIONALLICENCE 700 1- Friesen Henry Department of Physiology, University of Manitoba, R3E 0W5, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Wagner Graham Department of Physiology, University of Western Ontario, N6A 5C1, London, Ontario, Canada aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 283-290 0302-766X 267:2<283 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer