caa a22 4500 46908281X CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302959 doi (NATIONALLICENCE)springer-10.1007/BF00302959 The alpha-subunit of glycoprotein hormones exists in the prolactin secretory granules of the bullforg ( Rana catesbeiana ) pituitary gland [Elektronische Daten] [Shigeyasu Tanaka, Fumihiko Mizutani, Kazutoshi Yamamoto, Sakaé Kikuyama, Kazumasa Kurosumi] Summary: Our recent finding that the number of immunoreactive α-subunit cells was invariably greater than the total number of immunoreactive gonadotropin (GTH) and thyrotropin (TSH) cells in the bullfrog (Rana catesbeiana) pituitary gland raises the possibility that the α-subunit also exists in pituitary cells other than GTH and TSH cells. The present study demonstrates that there are a considerable number of immunoreactive prolactin (PRL) cells that are also stained with antibody against the α-subunit when adjacent sections are immunocytochemically examined. Neither immunoreactive growth hormone nor adrenocorticotropin cells are stained with the antibody against the α-subunit. The specificity of the antibody against the α-subunit and of that against PRL was demonstrated by preabsorption test, non-competitive binding test, and immunoblot analysis. Double-immunolabeling with gold particles of different sizes for the α-subunit and PRL revealed that most of the immunolabeled PRL-secretory granules are also labeled with the α-subunit antibody. The gold particles indicating the presence of the α-subunit were mostly found in the peripheral zone of the secretory granules. Springer-Verlag, 1992 α-Subunit nationallicence Pituitary glycoprotein hormone nationallicence PRL cell nationallicence Pars distalis nationallicence Colocalization nationallicence Immunocytochemistry nationallicence Bullfrog, Rana catesbeiana (Anura) nationallicence Tanaka Shigeyasu Department of Morphology, Institute of Endocrinology, Gunma University, Showa-machi 3-39-15, 371, Maebashi, Japan aut Mizutani Fumihiko Biological Institute, Faculty of Science, Niigata University, 950-21, Niigata, Japan aut Yamamoto Kazutoshi Department of Biology, School of Education, Waseda University, 169-50, Tokyo, Japan aut Kikuyama Sakaé Department of Biology, School of Education, Waseda University, 169-50, Tokyo, Japan aut Kurosumi Kazumasa Department of Morphology, Institute of Endocrinology, Gunma University, Showa-machi 3-39-15, 371, Maebashi, Japan aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 223-231 0302-766X 267:2<223 1992 267 441 https://doi.org/10.1007/BF00302959 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302959 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Shigeyasu Department of Morphology, Institute of Endocrinology, Gunma University, Showa-machi 3-39-15, 371, Maebashi, Japan aut NATIONALLICENCE 700 1- Mizutani Fumihiko Biological Institute, Faculty of Science, Niigata University, 950-21, Niigata, Japan aut NATIONALLICENCE 700 1- Yamamoto Kazutoshi Department of Biology, School of Education, Waseda University, 169-50, Tokyo, Japan aut NATIONALLICENCE 700 1- Kikuyama Sakaé Department of Biology, School of Education, Waseda University, 169-50, Tokyo, Japan aut NATIONALLICENCE 700 1- Kurosumi Kazumasa Department of Morphology, Institute of Endocrinology, Gunma University, Showa-machi 3-39-15, 371, Maebashi, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 223-231 0302-766X 267:2<223 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer