caa a22 4500 469082828 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302964 doi (NATIONALLICENCE)springer-10.1007/BF00302964 Atrial natriuretic peptide (ANP): a study of ANP and its mRNA in cardiocytes, and of plasma ANP levels in non-obese diabetic mice [Elektronische Daten] [Hiroharu Mifune, Syusaku Suzuki, Junichi Honda, Yuta Kobayashi, Yasutaka Noda, Yoshihiro Hayashi, Koshi Mochizuki] Summary: Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group. (P<0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P<0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P<0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group. Springer-Verlag, 1992 Atrial natriuretic peptides nationallicence mRNA nationallicence Diabetes, type I nationallicence Immunohistochemistry nationallicence Morphometry nationallicence Mouse (NOD) nationallicence Mifune Hiroharu Institute of Animal Experiment, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830, Fukuoka, Japan aut Suzuki Syusaku Institute of Laboratory Animal Sciences, Faculty of Medicine, Kagoshima University, Kagoshima, Japan aut Honda Junichi Department of Internal Medicine, Kurume University School of Medicine, Fukuoka, Japan aut Kobayashi Yuta Department of Pharmacology, Shimane Medical University, Shimane, Japan aut Noda Yasutaka Institute of Animal Experiment, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830, Fukuoka, Japan aut Hayashi Yoshihiro Laboratory of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Tokyo, Japan aut Mochizuki Koshi Laboratory of Biomedical Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 267-272 0302-766X 267:2<267 1992 267 441 https://doi.org/10.1007/BF00302964 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302964 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mifune Hiroharu Institute of Animal Experiment, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 700 1- Suzuki Syusaku Institute of Laboratory Animal Sciences, Faculty of Medicine, Kagoshima University, Kagoshima, Japan aut NATIONALLICENCE 700 1- Honda Junichi Department of Internal Medicine, Kurume University School of Medicine, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kobayashi Yuta Department of Pharmacology, Shimane Medical University, Shimane, Japan aut NATIONALLICENCE 700 1- Noda Yasutaka Institute of Animal Experiment, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hayashi Yoshihiro Laboratory of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Mochizuki Koshi Laboratory of Biomedical Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 267-272 0302-766X 267:2<267 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer