caa a22 4500 469082879 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302977 doi (NATIONALLICENCE)springer-10.1007/BF00302977 Hutson James Department of Cell Biology and Anatomy, Texas Tech University Health Sciences Center, 79430, Lubbock, TX, USA aut Development of cytoplasmic digitations between Leydig cells and testicular macrophages of the rat [Elektronische Daten] [James Hutson] Summary: Testicular macrophages and Leydig cells from adult animals are known to be functionally coupled. For example, secreted products from macrophages stimulate testosterone secretion by Leydig cells. In adult rat testes, structural coupling also exists between these cells. This coupling consists of cytoplasmic projections from Leydig cells located within cytoplasmic invaginations of macrophages. Although macrophages are known to exist in the testis in immature animals, it is not known when these digitations develop. The purpose of the present study was to determine whether the time of their development coincides with known maturational events that occur in Leydig cells, particularly during the peripubertal period. Testes from rats at 20, 30 and 40-days-of-age as well as testes from mature rats weighing more than 500 gm were prepared for ultrastructural analysis. It was found that digitations form between 20 and 30-days-of-age. These structures varied from simple tubular projections to complicated branched structures, suggesting that digitations are more than simple invaginations of microvilli into coated vesicles as previously described. Subplasmalemmal linear densities were also observed within macrophages juxtaposed to Leydig cells. Collagen was commonly observed between macrophages and Leydig cells in animals 20 days old. These studies demonstrate that although macrophages are present in the testis in maximal numbers at 20 days-of-age, they do not form junctions with Leydig cells until day 30. This is just prior to the major increase in secretory activity of rat Leydig cells that occurs during puberty. Springer-Verlag, 1992 Leydig cells nationallicence Macrophages nationallicence Development, ontogenic nationallicence Electron microscopy nationallicence Testis nationallicence (Rat Wistar) nationallicence Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 385-389 0302-766X 267:2<385 1992 267 441 https://doi.org/10.1007/BF00302977 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302977 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hutson James Department of Cell Biology and Anatomy, Texas Tech University Health Sciences Center, 79430, Lubbock, TX, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 385-389 0302-766X 267:2<385 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer