caa a22 4500 469082887 CHVBK 20180323132955.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00302957 doi (NATIONALLICENCE)springer-10.1007/BF00302957 Sulfhydryl oxidase immunoreactivity in seminiferous tubules of infertile men [Elektronische Daten] [M. Bergmann, G. Aumüller, J. Seitz, E. Nieschlag] Summary: Sulfhydryl oxidase (SOx) immunoreactivity was investigated in the seminiferous epithelium of human biopsy material from the testes of 33 adult men with disturbed fertility. SOx immunoreactivity was expressed in normal seminiferous epithelium in type-A spermatogonia (27±4% of all spermatogonia) (n=4), in spermatocytes and round spermatids. Mature spermatozoa as well as Sertoli cells were unlabelled. within the interstitium, Leydig cells were immunopositive. In biopsies of oligozoospermic men showing hypospermatogenesis (n=24), an increase in labelled spermatogonia up to more than 90% was observed in biopsies, where seminiferous epithelia revealed only spermatogonia and Sertoli cells. Within the group of oligozoospermic patients there was a significant increase of labelled spermatogonia from 43±13% (>20 mill/ejaculate) (n=7) to 55±16% ( 20 and >20 mill/ejaculate) (n=6) to 68±8% (<5 mill/ejaculate) (n=11) and a significant (P=0.01) decrease of score count from 7.0±2.7 to 2.0±1.8. In this group the increase of labelled spermatogonia was correlated with sperm concentrations in the ajaculate (correlation coefficient: r=-0.6). In biopsies of azoospermic patients showing maturation arrest at the level of spermatocytes or spermatids (n=5) the percentage of labelled spermatogonia was within the range of 24% to 59%. Immunoreactivity in Sertoli cells was only found in single degenerating cells and in tubules showing Sertoli Cell Only Syndrome (SCO) without lumen formation. Sertoli cells within immature seminiferous cords were immunonegative, indicating that Sertoli cell SOx immunoreactivity is rather a sign of physiological alterations in degenerating cells than dependent on the stage of differentiation. Leydig cells did not show changes of immunoreactivity in any biopsy. It is concluded that SOx expression in spermatogonia may serve as a marker for spermatogenic efficiency. Springer-Verlag, 1992 Testis nationallicence Sulfhydryl oxidase nationallicence Hypospermatogenesis nationallicence Sertoli cell integrity nationallicence Immunocytochemistry nationallicence Man nationallicence Bergmann M. Institut für Anatomie der Universität, Vesaliusweg 2-4, W-4400, Münster, Germany aut Aumüller G. Institut für Anatomie und Zellbiologie der Universität, W-3550, Marburg, Germany aut Seitz J. Institut für Anatomie und Zellbiologie der Universität, W-3550, Marburg, Germany aut Nieschlag E. Institut für Reproduktionsmedizin der Universität, Steinfurter Strasse 107, W-4400, Münster, Germany aut Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 209-214 0302-766X 267:2<209 1992 267 441 https://doi.org/10.1007/BF00302957 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302957 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bergmann M. Institut für Anatomie der Universität, Vesaliusweg 2-4, W-4400, Münster, Germany aut NATIONALLICENCE 700 1- Aumüller G. Institut für Anatomie und Zellbiologie der Universität, W-3550, Marburg, Germany aut NATIONALLICENCE 700 1- Seitz J. Institut für Anatomie und Zellbiologie der Universität, W-3550, Marburg, Germany aut NATIONALLICENCE 700 1- Nieschlag E. Institut für Reproduktionsmedizin der Universität, Steinfurter Strasse 107, W-4400, Münster, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 267/2(1992-02-01), 209-214 0302-766X 267:2<209 1992 267 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer