caa a22 4500 469082976 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381894 doi (NATIONALLICENCE)springer-10.1007/BF00381894 Haller C. Department of Anatomical Sciences, University of Queensland, 4072, Australia aut Evidence for the coexistence of serotonin and calcitonin gene-related peptide at the subcellular level in neuroepithelial bodies in the lung of a marsupial, Isoodon macrourus [Elektronische Daten] [C. Haller] Summary: The coexistence of serotonin and calcitonin gene-related peptide (CGRP) in neuroepithelial bodies of the bandicoot, Isoodon macrourus, has been examined using immunocytochemistry at the light- and electronmicroscope levels. The avidin-biotin technique of antigen localisation was used initially to identify serotonin-like and CGRP-like immunoreactivity (-LI). Serotonin-LI and CGRP-LI were found in neuroepithelial cells in the lungs of 30-day-old bandicoots. CGRP-LI could also be demonstrated in nerve fibres associated with some neuroepithelial bodies. The protein A-gold technique of antigen localisation was used to label neuroepithelial cells and nerve fibres at the subcellular level. Serotonin-LI and CGRP-LI were observed in the same dense-cored vesicles of most neuroepithelial cells; however, some neuroepithelial cells were shown to possess serotonin-LI without CGRP-LI. Nerve fibres immediately adjacent to neuroepithelial bodies exhibited mainly CGRP-LI. These results show that serotonin-LI and CGRP-LI are present in neuroepithelial cells of the bandicoot in the same secretory vesicles. This pattern of co-localisation may reflect co-ordinated or synergistic actions of these two neuroactive substances. Springer-Verlag, 1992 Neuroepithelial bodies nationallicence Lungs nationallicence Neuropeptide coexistence nationallicence Neuropeptide immunocytochemistry nationallicence Isoodon macrourus (Marsupialia) nationallicence Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 199-203 0302-766X 270:1<199 1992 270 441 https://doi.org/10.1007/BF00381894 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381894 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Haller C. Department of Anatomical Sciences, University of Queensland, 4072, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 199-203 0302-766X 270:1<199 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer