caa a22 4500 469082984 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381883 doi (NATIONALLICENCE)springer-10.1007/BF00381883 Ovarian innervation develops before initiation of folliculogenesis in the rat [Elektronische Daten] [Sasha Malamed, Jean Gibney, Sergio Ojeda] Summary: Sympathetic neurotransmitters have been shown to be present in the ovary of the rat during early postnatal development and to affect steroidogenesis before the ovary becomes responsive to gonadotropins, and before the first primordial follicles are formed. This study was undertaken to determine if development of the ovarian innervation is an event that antedates the initiation of folliculogenesis in the rat, Rattus norvegicus. Serial sections of postnatal ovaries revealed a negligible frequency of follicles 24 h after birth (about 1 primordial follicle per ovary). Twelve hours later there were about 500 follicles per ovary, a number that more than doubled to about 1300 during the subsequent 12 h, indicating that an explosive period of follicular differentiation occurs between the end of postnatal days 1 and 2. Electron microscopy demonstrated that before birth the ovaries are already innervated by fibers containing clear and dense-core vesicles. Immunohistochemistry performed on either fetal (day 19) or newborn (less than 15h after birth) ovaries showed the presence of catecholaminergic nerves, identified by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. While some of these fibers innervate blood vessels, others are associated with primordial ovarian cells, thereby suggesting their participation in non-vascular functions. Since prefollicular ovaries are insensitive to gonadotropins, the results suggest that the developing ovary becomes subjected to direct neurogenic influences before it acquires responsiveness to gonadotropins. Springer-Verlag, 1992 Ovarian nerves nationallicence Development nationallicence Folliculogenesis nationallicence Tyrosine hydroxylase nationallicence Immunohistochemistry nationallicence Electron microscopy nationallicence Rat (Sprague Dawley) nationallicence Malamed Sasha Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, 08854, Piscataway, New Jersey, USA aut Gibney Jean Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, 08854, Piscataway, New Jersey, USA aut Ojeda Sergio Division of Neuroscience, Oregon Regional Primate Research Center, 505 N.W. 185th Avenue Beaverton, 97006, OR, USA aut Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 87-93 0302-766X 270:1<87 1992 270 441 https://doi.org/10.1007/BF00381883 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381883 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malamed Sasha Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, 08854, Piscataway, New Jersey, USA aut NATIONALLICENCE 700 1- Gibney Jean Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, 08854, Piscataway, New Jersey, USA aut NATIONALLICENCE 700 1- Ojeda Sergio Division of Neuroscience, Oregon Regional Primate Research Center, 505 N.W. 185th Avenue Beaverton, 97006, OR, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 87-93 0302-766X 270:1<87 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer