caa a22 4500 46908300X CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381878 doi (NATIONALLICENCE)springer-10.1007/BF00381878 Ultrastructural evidence for multiple mucous domains in frog olfactory epithelium [Elektronische Daten] [Bert Menco, Albert Farbman] Summary: This study showed that the olfactory mucus is a highly structured extracellular matrix. Several olfactory epithelial glycoconjugates in the frog Rana pipiens were localized ultrastructurally using rapid-freeze, freeze-substitution and post-embedding (Lowicryl K11M) immunocytochemistry. Two of these conjugates were obtained from membrane preparations of olfactory cilia, the glycoproteins gp95 and olfactomedin. The other conjugates have a carbohydrate group which in the olfactory bulb appears to be mostly on neural cell-adhesion molecules (N-CAMs); in the olfactory epithelium this carbohydrate is present on more molecules. Localization of the latter conjugates was determined with monoclonal antibodies 9-OE and 5-OE. Ultrastructurally all antigens localized in secretory granules of apical regions of frog olfactory supporting cells and in the mucus overlying the epithelial surface, where they all had different, but partly overlapping, distributions. Monoclonal antibody 18.1, to gp95, labeled the mucus throughout, whereas poly- and monoclonal anti-olfactomedin labeled a deep mucous layer surrounding dendritic endings, proximal parts of cilia, and supporting cell microvilli. Labeling was absent in the superficial mucous layer, which contained the distal parts of the olfactory cilia. Monoclonal antibody 9-OE labeled rather distinct areas of mucus. These areas sometimes surrounded dendritic endings and olfactory cilia. Monoclonal antibody 5-OE labeled membranes of dendritic endings and cilia, and their glycocalyces, and also dendritic membranes. Springer-Verlag, 1992 Cilia nationallicence Microvilli nationallicence Secretory granules nationallicence Mucus nationallicence Freeze-substitution nationallicence Immunocytochemistry nationallicence Rana pipiens (Anura) nationallicence Menco Bert Department of Neurobiology and Physiology, Northwestern University, O.T. Hogan Hall, 60208-3520, Evanston, IL, USA aut Farbman Albert Department of Neurobiology and Physiology, Northwestern University, O.T. Hogan Hall, 60208-3520, Evanston, IL, USA aut Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 47-56 0302-766X 270:1<47 1992 270 441 https://doi.org/10.1007/BF00381878 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381878 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menco Bert Department of Neurobiology and Physiology, Northwestern University, O.T. Hogan Hall, 60208-3520, Evanston, IL, USA aut NATIONALLICENCE 700 1- Farbman Albert Department of Neurobiology and Physiology, Northwestern University, O.T. Hogan Hall, 60208-3520, Evanston, IL, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 47-56 0302-766X 270:1<47 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer