caa a22 4500 469083018 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381890 doi (NATIONALLICENCE)springer-10.1007/BF00381890 Immuno-electron-microscopic localization of laminin and collagen type IV in normal and denervated tooth pulp of the cat [Elektronische Daten] [K. Fried, M. Risling, L. Edwall, L. Olgart] Summary: The distribution of laminin-like immunoreactivity in adult normal and denervated cat mandibular tooth pulps was studied by the use of fluorescence microscopy and pre-embedding immunogold electron microscopy. Immunoreactivity to collagen IV was also assessed in order to distinguish basement membranes. In normal pulps, light-microscope laminin-like immunoreactivity was strong along blood vessels and Schwann cell sheaths, and a faint immunoreactivity was seen also in the odontoblast layer. Electron microscopy confirmed the laminin-like immunoreactivity of endothelial and Schwann cell basement membranes at all pulpal levels. In the odontoblast layer and the predentine, nerve-like structures lacking basement membranes but possessing strong membrane laminin-like immunoreactivity were encountered. In addition, a clear-cut laminin-like immunoreactivity of plasma membranes of the somata and processes of odontoblasts was seen. Observations on denervated pulps as well as pulps in which nerve regeneration had taken place did not reveal any changes in the pattern of laminin-immunoreactivity in basement membranes or odontoblasts. Distribution of collagen IV-like immunoreactivity was very similar to laminin-like immunoreactivity in basement membranes of blood vessels and Schwann cells, and appeared unaffected by denervation. The odontoblasts and nerve-like profiles in the odontoblast layer were devoid of collagen IV-like immunoreactivity. We propose that odontoblast-associated laminin could be of significance as guidance for regenerating terminal pulpal nerve fibers to appropriate targets. Springer-Verlag, 1992 Dental pulp nationallicence Laminin nationallicence Collagen IV nationallicence Odontoblast nationallicence Nerve regeneration nationallicence Immunocytochemistry nationallicence Cat nationallicence Fried K. Department of Anatomy, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut Risling M. Department of Anatomy, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut Edwall L. Department of Pharmacology, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut Olgart L. Department of Pharmacology, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 157-164 0302-766X 270:1<157 1992 270 441 https://doi.org/10.1007/BF00381890 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381890 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fried K. Department of Anatomy, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 700 1- Risling M. Department of Anatomy, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 700 1- Edwall L. Department of Pharmacology, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 700 1- Olgart L. Department of Pharmacology, Karolinska Institutet, POB 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 157-164 0302-766X 270:1<157 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer