caa a22 4500 469083077 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381893 doi (NATIONALLICENCE)springer-10.1007/BF00381893 Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation [Elektronische Daten] [Peter van der Ven, Gert Schaart, Paul Jap, Rob Sengers, Ad Stadhouders, Francs Ramaekers] Summary: This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and structural organization of the intermediate filament proteins desmin and vimentin as well as the sarcomeric constituents α-actin, α-actinin, nebulin, myosin and especially titin during myofibrillogenesis in vitro, were studied by means of indirect immunofluorescence assays. The proliferating myoblasts contained both desmin and vimentin, α-actinin and the filamentous form of actin. Shortly after the change of medium, expression of titin, sarcomeric myosin and skeletal muscle α-actin was found in mononuclear cells in a diffuse, filamentous (titin, myosin, α-actin) or punctate (titin, myosin) pattern. Four to 10 days after the medium change, mature myotubes showed desmin, titin, α-actinin, nebulin, sarcomeric myosin and actin cross-striations, while vimentin was no longer detected. We conclude that human skeletal muscle cell cultures are an appropriate model system to study the molecular basis of myofibrillogenesis. Especially the presence of desmin in a striated fashion points to a high degree of maturation of the muscle cell cultures. Springer-Verlag, 1992 Skeletal muscle cells nationallicence Myofibrillogenesis nationallicence Intermediate filaments nationallicence Titin nationallicence Nebulin nationallicence Myosin nationallicence α-Actinin nationallicence Human nationallicence van der Ven Peter Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut Schaart Gert Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands aut Jap Paul Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut Sengers Rob Department of Pediatrics, University of Nijmegen, The Netherlands aut Stadhouders Ad Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut Ramaekers Francs Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands aut Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 189-198 0302-766X 270:1<189 1992 270 441 https://doi.org/10.1007/BF00381893 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381893 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van der Ven Peter Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Schaart Gert Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands aut NATIONALLICENCE 700 1- Jap Paul Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Sengers Rob Department of Pediatrics, University of Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Stadhouders Ad Department of Cell Biology and Histology, University of Nijmegen, PB Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Ramaekers Francs Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 189-198 0302-766X 270:1<189 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer