caa a22 4500 46908314X CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00381873 doi (NATIONALLICENCE)springer-10.1007/BF00381873 Immunogold labelling of the cytoplasmic estradiol receptor in resting porcine endometrium [Elektronische Daten] [Walter Sierralta, Hubert Thole] Summary: Serial sections of resting porcine endometrium were analyzed with the monoclonal antibody 13H2 using goat antimouse IgG/5 nm gold as secondary reagent or with either polyclonal antibodies from goat #402 or the rat monoclonal antibody H222, both in combination with protein G/12 nm gold. A modestly higher labelling of nuclei than of cytoplasm was seen only with the monoclonal antibody H222. Polyclonal #402 and monoclonal 13H2 showed fewer attachments over nuclear than over cytoplasmic areas. The highest densities of attachment and of predominantly cytoplasmic labelling were obtained with the monoclonal antibody 13H2. The results confirm the earlier assumption of a restricted accessiblity of estradiol receptor in the cytoplasm of resting cells for immunoreagents. Springer-Verlag, 1992 Estradiol receptor nationallicence Endometrium nationallicence Ovariectomy nationallicence Immunohistochemistry nationallicence Ultrastructure nationallicence Pig nationallicence Sierralta Walter Max-Planck-Institut für Experimentelle Endokrinologie, P.O. Box 610309, W-3000, Hannover 61, Federal Republic of Germany aut Thole Hubert Max-Planck-Institut für Experimentelle Endokrinologie, P.O. Box 610309, W-3000, Hannover 61, Federal Republic of Germany aut Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 1-6 0302-766X 270:1<1 1992 270 441 https://doi.org/10.1007/BF00381873 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00381873 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sierralta Walter Max-Planck-Institut für Experimentelle Endokrinologie, P.O. Box 610309, W-3000, Hannover 61, Federal Republic of Germany aut NATIONALLICENCE 700 1- Thole Hubert Max-Planck-Institut für Experimentelle Endokrinologie, P.O. Box 610309, W-3000, Hannover 61, Federal Republic of Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/1(1992-10-01), 1-6 0302-766X 270:1<1 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer