caa a22 4500 469083158 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645060 doi (NATIONALLICENCE)springer-10.1007/BF00645060 Immuno-electron-microscopic localization of basic fibroblast growth factor in the dystrophic mdx mouse masseter muscle [Elektronische Daten] [Seiji Matsuda, Junzo Desaki, Hiroko Fujita, Nobuaki Okumura, Masahiro Sakanaka] Summary: The localization of basic fibroblast growth factor (bFGF)-like immunoreactivity in the masseter muscle of dystrophic mdx mice on postnatal day 28 was investigated by immunoblot analysis and electron microscopy. Crude homogenate of the masseter muscle, when subjected to immunoblotting with a bFGF antiserum, exhibited a main band with the same molecular weight (18 kDa) as bovine bFGF. By electron microscopy, bFGF immunoreactivity was detected in small regenerating myocytes; the smaller cells were the premature myocytes, the most intense staining was the immunoreactivity within the cytoplasm. Putative precursors of the muscle cells with a few myofilaments, which were most intensely labeled with anti-bFGF, contacted each other and possibly developed into multinucleated myocytes through cell fusion. Mature myocytes with densely packed myofilaments and peripherally located nuclei did not exhibit bFGF immunoreactivity; they formed myoneural junctions with motor nerve endings immunoreactive for bFGF. Early differentiating myocytes with intense bFGF-like immunoreactivity did not make contact with immunoreactive nerve terminals. Degenerating large myocytes with a limited number of distorted and/or disrupted myofilaments exhibited electron-dense deposits in the cristae of mitochondria; these deposits were not abolished by immunoadsorption control experiments. Thus, the cell-size-dependent decrease in bFGF immunoreactivity in regenerating but not in degenerating myocytes provides a morphological basis for an autoregulatory role of bFGF in muscle regeneration. This study suggests that neuronal bFGF is not involved in initial muscle regeneration in the dystrophic mdx mouse. Springer-Verlag, 1992 Basic fibroblast growth factor nationallicence Regeneration nationallicence Degeneration nationallicence Immunohistochemistry nationallicence Electron microscopy nationallicence Masseter muscle nationallicence Myoneural junction nationallicence Mouse (dystrophic mdx) nationallicence Matsuda Seiji Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut Desaki Junzo Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut Fujita Hiroko Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut Okumura Nobuaki Department of Biochemistry, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut Sakanaka Masahiro Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 569-576 0302-766X 270:3<569 1992 270 441 https://doi.org/10.1007/BF00645060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuda Seiji Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut NATIONALLICENCE 700 1- Desaki Junzo Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut NATIONALLICENCE 700 1- Fujita Hiroko Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut NATIONALLICENCE 700 1- Okumura Nobuaki Department of Biochemistry, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut NATIONALLICENCE 700 1- Sakanaka Masahiro Department of Anatomy, Ehime University School of Medicine, Singenobu, 791-02, Ehime, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 569-576 0302-766X 270:3<569 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer