caa a22 4500 469083166 CHVBK 20180323132956.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645046 doi (NATIONALLICENCE)springer-10.1007/BF00645046 Extra-Golgi pathway of an acrosomal antigen during spermiogenesis in the rat [Elektronische Daten] [I. Tanii, K. Toshimori, S. Araki, C. Oura] Summary: A monoclonal antibody (MC41) was produced that specifically recognizes a sperm acrosomal antigen of approximately 165000 dalton in the rat. Rat testis was examined using a pre-embedding immunoperoxidase technique to reveal the pathway of the MC41 antigen to the acrosome during spermiogenesis. The MC41 immunoreaction appeared in several organelles of spermatids in a stage-specific manner: (1) in the endoplasmic reticulum (ER) throughout spermiogenesis, (2) in the outer acrosomal membrane from steps 9 to 19, (3) as a weak immunoreaction in the vesicular structures in the acrosomal matrix from steps 11 to 17, and (4) as a strong immunoreaction in the acrosomal matrix especially at the terminal step of spermiogenesis (step 19). However, no immunoreaction was observed in the Golgi region throughout spermiogenesis. These results suggest that the pathway of the MC41 antigen leads firstly from the ER to the outer acrosomal membrane and secondly to the acrosomal matrix. This pathway does not involve the Golgi apparatus and is referred to as the "extra-Golgi pathway”. Springer-Verlag, 1992 Intra-acrosomal protein nationallicence Acrosome development nationallicence Extra-Golgi pathway nationallicence Spermiogenesis nationallicence Immunoelectron microscopy nationallicence Rat (Wistar) nationallicence Tanii I. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut Toshimori K. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut Araki S. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut Oura C. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 451-457 0302-766X 270:3<451 1992 270 441 https://doi.org/10.1007/BF00645046 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645046 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanii I. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Toshimori K. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Araki S. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Oura C. Department of Anatomy, Miyazaki Medical College, Kihara 5200 Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 451-457 0302-766X 270:3<451 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer