caa a22 4500 469083220 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645056 doi (NATIONALLICENCE)springer-10.1007/BF00645056 Immunohistochemical localisation of natriuretic peptides in the brains and hearts of the spiny dogfish Squalus acanthias and the Atlantic hagfish Myxine glutinosa [Elektronische Daten] [John Donald, Archie Vomachka, David Evans] Summary: The avidin-biotin peroxidase technique was used to determine the distribution of natriuretic peptides in the hearts and brains of the dogfishSqualus acanthias and the Atlantic hagfishMyxine glutinosa. Three antisera were used: one raised against porcine brain natriuretic peptide which cross-reacts with atrial natriuretic and C-type natriuretic peptides (termed natriuretic peptide-like immunoreactivity); the second raised against porcine brain natriuretic peptide which cross-reacts with C-type natriuretic peptide, but not with atrial natriuretic peptide (termed porcine brain natriuretic peptide-like immunoreactivity); and the third raised against rat atrial natriuretic peptide (termed rat atrial natriuretic peptide-like immunoreactivity). Only natriuretic peptide-like immunoreactivity was observed in the heart ofS. acanthias which was most likely due to the antiserum cross-reacting with C-type natriuretic peptide. No immunoreactivity was found in theM. glutinosa heart. In the brain ofS. acanthias, natriuretic peptide-like immunoreactive fibres were located in many areas of the telencephalon, diencephalon, mesencephalon, rhombencephalon, and spinal cord. Extensive immunoreactivity was observed in the hypothalamo-hypophyseal tract and the neurointermediate lobe of the hypophysis. Natriuretic peptide-like immunoreactive perikarya were found in ventromedial regions of the telencephalon and in the nucleus preopticus. Most perikarya had short, thick processes which extended toward the ventricle. Another group of perikarya was observed in the rhombencephalon. Porcine brain natriuretic peptide-like immunoreactive fibres were observed in the telencephalon, diencephalon, mesencephalon, and rhombencephalon, but perikarya were only present in the preoptic area. In theM. glutinosa brain, natriuretic peptide-like immunoreactive fibres were present in all regions. Immunoreactive perikarya were observed in the pallium, primordium hippocampi, pars ventralis thalami, pars dorsalis thalami, nucleus diffusus hypothalami, nucleus profundus, nucleus tuberculi posterioris, and nucleus ventralis tegmenti. Procine brain natriuretic peptide-like immunoreactive perikarya and fibres had a similar, but less abundant distribution than natriuretic peptide-like immunoreactive structures. Although the chemical structures of natriuretic peptides in the brains of dogfish and hagfish are unknown, these observations show that a component of the natriuretic peptide complement is similar to porcine brain natriuretic peptide or porcine C-type natriuretic peptide. The presence of natriuretic peptides in the brain suggest they could be important neuromodulators and/or neurotransmitters. Furthermore, there appears to be divergence in the structural forms of natriuretic peptides in the hearts and brains of dogfish and hagfish. Springer-Verlag, 1992 Atrial natriuretic peptide nationallicence Brain natriuretic peptide nationallicence C-type natriuretic peptide nationallicence Heart nationallicence Brain, vertebrate nationallicence Immunohistochemistry nationallicence Squalus acanthias (Elasmobranchii) nationallicence Myxine glutinosa (Cyclostomata) nationallicence Donald John Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut Vomachka Archie Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut Evans David Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 535-545 0302-766X 270:3<535 1992 270 441 https://doi.org/10.1007/BF00645056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donald John Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut NATIONALLICENCE 700 1- Vomachka Archie Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut NATIONALLICENCE 700 1- Evans David Mount Desert Island Biological Laboratory, 04672, Salsbury Cove, ME, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 535-545 0302-766X 270:3<535 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer