caa a22 4500 469083247 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645049 doi (NATIONALLICENCE)springer-10.1007/BF00645049 Glutathione and γ-glutamyl transpeptidase are differentially distributed in the olfactory mucosa of rats [Elektronische Daten] [N. Rama Krishna, Marilyn Getchell, Suresh Tate, Frank Margolis, Thomas Getchell] Summary: Components of the γ-glutamyl cycle, including thiols, glutathione (GSH) and γ-glutamyl transpeptidase (γ-GT), were localized in the nasal mucosae of rats using histochemical and immunohistochemical methods. In olfactory mucosa, thiols were widely distributed, with intense staining in the mucociliary complex (MC), basal cells, acinar cells of Bowman's glands (BG), and olfactory nerve bundles, and with moderate staining in olfactory receptor neurons (ORNs). GSH was localized in MC, BG acinar cells, nerve bundles and, to a lesser extent, in ORNs. γ-GT immunoreactivity was restricted to the MC and to basolateral and apical membranes of BG acinar and duct cells. The basolateral membrane of BG acinar cells, located in close association with blood vessels and connective tissue, showed granule-like immunoreactivity. Inrespiratory mucosa, all three compounds were localized in the MC and acinar cells of respiratory glands (RG). In the MC, γ-GT immunoreactivity was associated primarily with brush borders of ciliated cells. Granular immunoreactivity was also apparent in the supranuclear region of RG acinar cells. These results demonstrate that components of the γ-glutamyl cycle are localized in olfactory and respiratory glands, and that they are secreted into the mucus, where they may mediate perireceptor events such as detoxification and/or solubilization of air-borne xenobiotics, toxicants and odorants. Springer-Verlag, 1992 Thiols nationallicence Glutathione nationallicence γ-Glutamyl transpeptidase nationallicence Olfactory epithelium nationallicence Respiratory epithelium nationallicence Perireceptor events nationallicence Rat (Sprague Dawley) nationallicence Rama Krishna N. Department of Physiology and Biophysics, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut Getchell Marilyn Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut Tate Suresh Department of Biochemistry, Cornell University Medical College, 10021, New York, NY, USA aut Margolis Frank Department of Neuroscience, Roche Institute of Molecular Biology, 07110, Nutley, NJ, USA aut Getchell Thomas Department of Physiology and Biophysics, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 475-484 0302-766X 270:3<475 1992 270 441 https://doi.org/10.1007/BF00645049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rama Krishna N. Department of Physiology and Biophysics, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Getchell Marilyn Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Tate Suresh Department of Biochemistry, Cornell University Medical College, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Margolis Frank Department of Neuroscience, Roche Institute of Molecular Biology, 07110, Nutley, NJ, USA aut NATIONALLICENCE 700 1- Getchell Thomas Department of Physiology and Biophysics, University of Kentucky College of Medicine, 40536, Lexington, KY, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 475-484 0302-766X 270:3<475 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer