caa a22 4500 469083263 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645053 doi (NATIONALLICENCE)springer-10.1007/BF00645053 Appearance and endocytic activity of epithelial cells from human efferent ducts in primary monolayer culture [Elektronische Daten] [S. Raczek, C. Yeung, L. Hertle, H. Schulze, T. Cooper] Summary: The culture of epithelial cells lining human efferent ducts, obtained from prostatic carcinoma patients, is described. Ciliated cells were observed to beat for at least one month on plastic. On pervious filters low cuboidal cells characterized the monolayers. Cells comprising monolayers over the filter were 5 to 9 μm in height whereas taller cells were found over the original fragments (14 μm). Some non-ciliated cells contained dark and light vacuoles, others were found to lack them. Both non-ciliated and ciliated cells maintained tight junctional complexes restricting the paracellular movement of horseradish peroxidase. Both types of cultured cells exhibited fluid-phase and adsorptive endocytosis from both apical and basal surfaces. It is reported for the first time that the monolayers form high resistance barriers (150 Ω cm2) that prevent the apical medium from draining to the basal compartment over 24 h. Springer-Verlag, 1992 Reproductive system, male nationallicence Ductuli efferentes nationallicence Epithelial cells nationallicence Cell culture nationallicence Monolayer cultures nationallicence Man nationallicence Raczek S. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut Yeung C. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut Hertle L. Institut für Urologie, Westfälische Wilhelms-Universität, Münster, Federal Republic of Germany aut Schulze H. Institut für Urologie, Ruhr Universität Bochum, Federal Republic of Germany aut Cooper T. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 513-519 0302-766X 270:3<513 1992 270 441 https://doi.org/10.1007/BF00645053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raczek S. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Yeung C. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hertle L. Institut für Urologie, Westfälische Wilhelms-Universität, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schulze H. Institut für Urologie, Ruhr Universität Bochum, Federal Republic of Germany aut NATIONALLICENCE 700 1- Cooper T. Institut für Reproduktionsmedizin, Westfälische Wilhelms-Universität Münster, Steinfurter Strasse 107, W-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 513-519 0302-766X 270:3<513 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer