caa a22 4500 469083271 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645061 doi (NATIONALLICENCE)springer-10.1007/BF00645061 Structural and enzymatic studies on the plasma membrane domains and sodium pump enzymes of absorptive epithelial cells in the avian lower intestine [Elektronische Daten] [T. Mayhew, V. Elbrønd, V. Dantzer, E. Skadhauge, O. Møller] Summary: The coprodaeum of the domestic hen maintained on a low-NaCl diet adapts by enhanced sodium transport. This study examines the adaptive response at the single cell and whole organ levels. Surface areas of apical (microvillous) and basolateral plasma membranes of columnar absorptive epithelial cells were estimated by use of ultrastructural stereology. The activities of succinic dehydrogenase (a mitochondrial enzyme) and ouabain-sensitive, potassium-dependent paranitrophenyl phosphatase (a sodium pump enzyme) were determined in tissue homogenates. Sodium, potassium-ATPase (pump enzyme) activity in cell membranes was localized by ultrastructural cytochemistry. Apical and basolateral membranes responded differently. In high-NaCl hens, the membrane signature of the average cell was 32 μm2 (apical), 932 μm2 (lateral) and 17 μm2 (basal). Cells from low-NaCl hens had more apical membrane (49 μm2 per cell) but essentially the same area of basolateral membrane. However, total surfaces per organ were greater for all membranes. Sodium pump enzymes were localized in basolateral membranes. Enzyme activities per unit mitochondrial volume and per unit basolateral membrane surface were higher in low-NaCl birds. These findings are discussed in the context of known mechanisms of transcellular sodium transport via apical ion channels and basolateral pumps. Springer-Verlag, 1992 Intestine nationallicence Sodium transport nationallicence Enterocyte nationallicence Plasma membranes nationallicence Stereology nationallicence Biochemistry nationallicence Cytochemistry nationallicence Domestic fowl nationallicence Mayhew T. Department of Human Morphology, Queen's Medical Centre, University of Nottingham, NG7 2UH, Nottingham, UK aut Elbrønd V. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut Dantzer V. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut Skadhauge E. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut Møller O. Department of Physiology, University of Aarhus, Denmark aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 577-585 0302-766X 270:3<577 1992 270 441 https://doi.org/10.1007/BF00645061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mayhew T. Department of Human Morphology, Queen's Medical Centre, University of Nottingham, NG7 2UH, Nottingham, UK aut NATIONALLICENCE 700 1- Elbrønd V. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut NATIONALLICENCE 700 1- Dantzer V. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut NATIONALLICENCE 700 1- Skadhauge E. Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark aut NATIONALLICENCE 700 1- Møller O. Department of Physiology, University of Aarhus, Denmark aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 577-585 0302-766X 270:3<577 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer