caa a22 4500 469083298 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645055 doi (NATIONALLICENCE)springer-10.1007/BF00645055 Structure and protein composition of sites of papillary muscle attachment to chordae tendineae in avian hearts [Elektronische Daten] [James Tidball, Karyl Andolina] Summary: Most cardiac myocytes transmit force across fasciae adherentes, specialized sites of cell-cell adhesion. However, some cardiac myocytes in papillary muscle terminate on collagenous connective tissue in the chordae tendineae. These papillary myotendinous junctions (MTJs) are specialized for force transmission from myocytes to extracellular matrix. In the present study, we compared structural molecules at papillary MTJs to those at fasciae adherentes and skeletal MTJs. By using indirect immunofluorescence, we found that papillary MTJs more closely resemble skeletal MTJs in their molecular composition in that they are enriched in talin, vinculin, integrin, and fibronectin. Zeugmatin and α-actinin, both components of fasciae adherentes, are absent from papillary MTJs. Although papillary MTJs and skeletal MTJs display strong similarities in structural protein composition, ultrastructural organization of the two junctions is different. Papillary MTJs display little folding of the junctional membrane and, according to morphological criteria, more closely resemble sites of thin filament-membrane association in smooth muscle than skeletal MTJs. Thus, papillary MTJs display a combination of structural characteristics described previously in skeletal and smooth muscles but exhibit few structural features observed previously in cardiac fasciae adherentes. Springer-Verlag, 1992 Muscle, cardiac nationallicence Muscle, striated, skeletal Cytoskeleton nationallicence Myotendinous junctions nationallicence Chordae tendineac nationallicence Domestic Fowl nationallicence Tidball James Department of Physiological Science, University of California, Los Angeles, 405 Hilgard Avenue, 90024-1527, Los Angeles, CA, USA aut Andolina Karyl Department of Physiological Science, University of California, Los Angeles, 405 Hilgard Avenue, 90024-1527, Los Angeles, CA, USA aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 527-533 0302-766X 270:3<527 1992 270 441 https://doi.org/10.1007/BF00645055 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645055 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tidball James Department of Physiological Science, University of California, Los Angeles, 405 Hilgard Avenue, 90024-1527, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Andolina Karyl Department of Physiological Science, University of California, Los Angeles, 405 Hilgard Avenue, 90024-1527, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 527-533 0302-766X 270:3<527 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer