caa a22 4500 469083301 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645063 doi (NATIONALLICENCE)springer-10.1007/BF00645063 Expression of mRNA of parathyroid hormone-related peptide in fetal bones of the rat [Elektronische Daten] [Rafik Karmali, Serge Schiffmann, Jean-Marie Vanderwinden, Geoffrey Hendy, Nicole Nys-DeWolf, Jacques corvilain, Pierre Bergmann, Jean-Jacques Vanderhaeghen] Summary: Previous studies have indicated that 19-dayold fetal long bones of the rat contain an adenylyl cyclase-stimulating activity antigenically related to parathyroid hormone-related peptide. To ascertain its origin, Northern blotting and in situ hybridization histochemistry were performed. Results demonstrate that mRNA of parathyroid hormone-related peptide is present in RNA extracted from fetal long bones of the rat and that cells responsible for its production are localized in the periosteum. These cells are not mature osteoblasts because they do not synthesize mRNA of osteocalcin. Thus the present study shows that parathyroid hormone-related peptide could be produced locally, at least in part, in the skeleton of fetal rats. Springer-Verlag, 1992 Parathyroid hormone-related peptide nationallicence Osteocalcin-mRNA nationallicence In situ hybridization histochemistry nationallicence Bone nationallicence Rat (Wistar) nationallicence Karmali Rafik Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut Schiffmann Serge Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut Vanderwinden Jean-Marie Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut Hendy Geoffrey Calcium Research Laboratory, McGill University, Montreal, Canada aut Nys-DeWolf Nicole Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut corvilain Jacques Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut Bergmann Pierre Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut Vanderhaeghen Jean-Jacques Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 597-600 0302-766X 270:3<597 1992 270 441 https://doi.org/10.1007/BF00645063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karmali Rafik Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- Schiffmann Serge Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- Vanderwinden Jean-Marie Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- Hendy Geoffrey Calcium Research Laboratory, McGill University, Montreal, Canada aut NATIONALLICENCE 700 1- Nys-DeWolf Nicole Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- corvilain Jacques Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- Bergmann Pierre Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 700 1- Vanderhaeghen Jean-Jacques Laboratory of Neuropathology, and Neuropeptides, Brugmann Hospital, Brussels, Belgium aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 597-600 0302-766X 270:3<597 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer